R2R3型MYB转录因子GhMYB109是棉花纤维发育所必需的。
The R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development.
作者信息
Pu Li, Li Qun, Fan Xiaoping, Yang Weicai, Xue Yongbiao
机构信息
Institute of Cotton Research, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, Shanxi Province, China.
出版信息
Genetics. 2008 Oct;180(2):811-20. doi: 10.1534/genetics.108.093070. Epub 2008 Sep 9.
Abstract
Cotton (Gossypium hirsutum L.) fibers are single highly elongated cells derived from the outer epidermis of ovules. A large number of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the process of fiber development. Here we examine the role of the cotton-fiber-specific R2R3 MYB gene GhMYB109 in cotton fiber development. Transgenic reporter gene analysis revealed that a 2-kb GhMYB109 promoter was sufficient to confirm its fiber-specific expression. Antisense-mediated suppression of GhMYB109 led to a substantial reduction in fiber length. Consistently, several genes related to cotton fiber growth were found to be significantly reduced in the transgenic cotton. Our results showed that GhMYB109 is required for cotton fiber development and reveal a largely conserved mechanism of the R2R3 MYB transcription factor in cell fate determination in plants.
摘要
棉花（陆地棉）纤维是源自胚珠外表皮的单个高度伸长的细胞。纤维分化和发育需要大量基因，但迄今为止，对于这些基因如何控制和调节纤维发育过程知之甚少。在此，我们研究了棉花纤维特异性R2R3 MYB基因GhMYB109在棉花纤维发育中的作用。转基因报告基因分析表明，一个2 kb的GhMYB109启动子足以证实其纤维特异性表达。反义介导的GhMYB109抑制导致纤维长度大幅缩短。同样，在转基因棉花中发现几个与棉花纤维生长相关的基因显著减少。我们的结果表明，GhMYB109是棉花纤维发育所必需的，并揭示了R2R3 MYB转录因子在植物细胞命运决定中很大程度上保守的机制。
相似文献
1
The R2R3 MYB transcription factor GhMYB109 is required for cotton fiber development.R2R3型MYB转录因子GhMYB109是棉花纤维发育所必需的。
Genetics. 2008 Oct;180(2):811-20. doi: 10.1534/genetics.108.093070. Epub 2008 Sep 9.
2
Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.).鉴定编码R2R3 MYB转录因子的GhMYB109，该转录因子在棉花（陆地棉）的纤维起始细胞和伸长的纤维中特异性表达。
Biochim Biophys Acta. 2003 Oct 20;1630(1):25-34. doi: 10.1016/j.bbaexp.2003.08.009.
3
GhMYB25-like: a key factor in early cotton fibre development.GhMYB25-like：早期棉花纤维发育的关键因素。
Plant J. 2011 Mar;65(5):785-97. doi: 10.1111/j.1365-313X.2010.04464.x. Epub 2011 Jan 14.
4
Genome-Wide Identification of R2R3-MYB Transcription Factors Regulating Secondary Cell Wall Thickening in Cotton Fiber Development.全基因组鉴定调控棉花纤维发育次生细胞壁增厚的 R2R3-MYB 转录因子。
Plant Cell Physiol. 2019 Mar 1;60(3):687-701. doi: 10.1093/pcp/pcy238.
5
Epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, GhHD-1.GhHD-1 介导的陆地棉表皮细胞分化的同源域亮氨酸拉链基因。
Plant J. 2012 Aug;71(3):464-78. doi: 10.1111/j.1365-313X.2012.05003.x. Epub 2012 May 28.
6
Functional analysis of the seed coat-specific gene GbMYB2 from cotton.棉属种子特异性基因 GbMYB2 的功能分析。
Plant Physiol Biochem. 2013 Dec;73:16-22. doi: 10.1016/j.plaphy.2013.08.004. Epub 2013 Aug 20.
7
Phosphorylation of WRKY16 by MPK3-1 is essential for its transcriptional activity during fiber initiation and elongation in cotton (Gossypium hirsutum).MPK3-1 对 WRKY16 的磷酸化在棉花（Gossypium hirsutum）纤维起始和伸长过程中对其转录活性至关重要。
Plant Cell. 2021 Aug 31;33(8):2736-2752. doi: 10.1093/plcell/koab153.
8
A R2R3-MYB transcription factor that is specifically expressed in cotton (Gossypium hirsutum) fibers affects secondary cell wall biosynthesis and deposition in transgenic Arabidopsis.一种在棉花（陆地棉）纤维中特异性表达的R2R3-MYB转录因子影响转基因拟南芥的次生细胞壁生物合成和沉积。
Physiol Plant. 2015 Jul;154(3):420-32. doi: 10.1111/ppl.12317. Epub 2015 Jan 26.
9
The MYB transcription factor GhMYB25 regulates early fibre and trichome development.MYB转录因子GhMYB25调控早期纤维和毛状体发育。
Plant J. 2009 Jul;59(1):52-62. doi: 10.1111/j.1365-313X.2009.03847.x. Epub 2009 Feb 26.
10
The cotton (Gossypium hirsutum) NAC transcription factor (FSN1) as a positive regulator participates in controlling secondary cell wall biosynthesis and modification of fibers.棉花（Gossypium hirsutum）NAC 转录因子（FSN1）作为一个正调控因子参与控制次生细胞壁生物合成和纤维的修饰。
New Phytol. 2018 Jan;217(2):625-640. doi: 10.1111/nph.14864. Epub 2017 Nov 6.
引用本文的文献
1
Duplicated Genes on Homologous Chromosomes Decipher the Dominant Epistasis of the Fiberless Mutant in Cotton.同源染色体上的重复基因解析棉花无纤维突变体的显性上位性
Biology (Basel). 2025 Aug 2;14(8):983. doi: 10.3390/biology14080983.
2
Pangenome analysis reveals yield- and fiber-related diversity and interspecific gene flow in Gossypium barbadense L.泛基因组分析揭示了海岛棉与产量和纤维相关的多样性及种间基因流动
Nat Commun. 2025 May 29;16(1):4995. doi: 10.1038/s41467-025-60254-x.
3
Genes Affecting Cotton Fiber Length: A Systematic Review and Meta-Analysis.影响棉花纤维长度的基因：系统综述与荟萃分析
Plants (Basel). 2025 Apr 12;14(8):1203. doi: 10.3390/plants14081203.
4
A high-resolution model of gene expression during Gossypium hirsutum (cotton) fiber development.陆地棉（棉花）纤维发育过程中基因表达的高分辨率模型。
BMC Genomics. 2025 Mar 6;26(1):221. doi: 10.1186/s12864-025-11360-z.
5
GhLPF1 Associated Network Is Involved with Cotton Lint Percentage Regulation Revealed by the Integrative Analysis of Spatial Transcriptome.通过空间转录组综合分析揭示的与棉绒百分比调控相关的GhLPF1关联网络
Adv Sci (Weinh). 2025 Apr;12(13):e2414175. doi: 10.1002/advs.202414175. Epub 2025 Feb 11.
6
Spatiotemporal transcriptome and metabolome landscapes of cotton fiber during initiation and early development.棉花纤维起始和早期发育过程中的时空转录组和代谢组图谱
Nat Commun. 2025 Jan 20;16(1):858. doi: 10.1038/s41467-025-55869-z.
7
Transcriptional regulation of phospholipid transport in cotton fiber elongation by GhMYB30D04-GhHD1 interaction complex.GhMYB30D04-GhHD1 互作复合物调控棉花纤维伸长过程中磷脂转运的转录调控。
J Integr Plant Biol. 2024 Nov;66(11):2431-2449. doi: 10.1111/jipb.13776. Epub 2024 Sep 17.
8
R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis.R2R3 MYB 转录因子 GhMYB201 通过细胞壁松弛和超长链脂肪酸合成促进棉花纤维伸长。
Int J Mol Sci. 2024 Sep 3;25(17):9559. doi: 10.3390/ijms25179559.
9
Sphingosine Promotes Fiber Early Elongation in Upland Cotton.鞘氨醇促进陆地棉纤维早期伸长。
Plants (Basel). 2024 Jul 21;13(14):1993. doi: 10.3390/plants13141993.
10
controls fiber initiation and early elongation by regulating ethylene signaling pathway in cotton ().通过调控棉花中的乙烯信号通路来控制纤维起始和早期伸长。
Mol Breed. 2024 May 16;44(6):38. doi: 10.1007/s11032-024-01477-6. eCollection 2024 Jun.
本文引用的文献
1
Analysis of gene expression in cotton fiber initials.棉花纤维起始细胞中的基因表达分析。
BMC Plant Biol. 2007 May 16;7:22. doi: 10.1186/1471-2229-7-22.
2
Accumulation of genome-specific transcripts, transcription factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton.异源四倍体棉花纤维细胞发育早期基因组特异性转录本、转录因子和植物激素调节因子的积累
Plant J. 2006 Sep;47(5):761-75. doi: 10.1111/j.1365-313X.2006.02829.x. Epub 2006 Aug 2.
3
Trichomes: different regulatory networks lead to convergent structures.植物毛状体：不同的调控网络导致趋同结构。
Trends Plant Sci. 2006 Jun;11(6):274-80. doi: 10.1016/j.tplants.2006.04.008. Epub 2006 May 11.
4
A global assembly of cotton ESTs.棉花表达序列标签的全球汇编。
Genome Res. 2006 Mar;16(3):441-50. doi: 10.1101/gr.4602906. Epub 2006 Feb 14.
5
Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation.转录组分析、分子生物学和生理学研究表明，乙烯在棉花纤维细胞伸长中起主要作用。
Plant Cell. 2006 Mar;18(3):651-64. doi: 10.1105/tpc.105.040303. Epub 2006 Feb 3.
6
Expression profiling identifies genes expressed early during lint fibre initiation in cotton.表达谱分析鉴定出棉花棉纤维起始早期表达的基因。
Plant Cell Physiol. 2006 Jan;47(1):107-27. doi: 10.1093/pcp/pci228. Epub 2005 Nov 7.
7
Developmental and gene expression analyses of a cotton naked seed mutant.棉花无籽突变体的发育及基因表达分析
Planta. 2006 Feb;223(3):418-32. doi: 10.1007/s00425-005-0098-7. Epub 2005 Oct 28.
8
Genome-wide analysis of S-Locus F-box-like genes in Arabidopsis thaliana.拟南芥中S位点类F盒基因的全基因组分析。
Plant Mol Biol. 2004 Dec;56(6):929-45. doi: 10.1007/s11103-004-6236-y. Epub 2005 Apr 7.
9
Two WD-repeat genes from cotton are functional homologues of the Arabidopsis thaliana TRANSPARENT TESTA GLABRA1 (TTG1) gene.棉花中的两个WD重复基因是拟南芥透明种皮光滑基因1（TTG1）的功能同源基因。
Plant Mol Biol. 2005 Jan;57(1):67-81. doi: 10.1007/s11103-004-6768-1.
10
The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation.棉花肌动蛋白1基因在纤维中功能性表达，并参与纤维伸长。
Plant Cell. 2005 Mar;17(3):859-75. doi: 10.1105/tpc.104.029629. Epub 2005 Feb 18.
Zotero 插件