伊洛前列素和己酮可可碱减轻兔模型骨骼肌缺血再灌注损伤。

Iloprost and pentoxifylline attenuate ischemia-reperfusion injury in skeletal muscle in rabbit model.

作者信息

Emrecan Bilgin, Tulukoğlu Engin, Bozok Sahin, Aksun Murat, Yağdi Serhan, Ozcan Ali Vefa, Saçar Mustafa, Gürbüz Ali

机构信息

Department of Cardiovascular Diseases, Medicine Faculty of Pamukkale University, Denizli, Turkey.

出版信息

Ulus Travma Acil Cerrahi Derg. 2008 Jul;14(3):182-7.

PMID:18781412

Abstract

BACKGROUND

We investigated the effects of iloprost and pentoxifylline on skeletal muscle ischemia-reperfusion injury in a rabbit model.

METHODS

Forty New Zealand white rabbits were grouped into four. In Group 1, iloprost was continuously infused starting half an hour before the reperfusion following a 2-hour ischemia formed by abdominal aortic occlusion, and it was continued during the 4-hour reperfusion period. Group 2 was treated with pentoxifylline, and Group 3 received saline solution. Group 4 was the sham group. Malondialdehyde levels and edema scores in gastrocnemius muscle were evaluated.

RESULTS

Edema score was significantly lower in Group 1 when compared with the control group (Group 1 vs Group 3, p=0.040; Group 2 vs Group 3, p=0.145; Group 1 vs Group 2, p=0.580). Malondialdehyde levels of the medicated groups were significantly lower when compared with the control group (Group 1: 60+/-11 nmol/g tissue, Group 2: 74+/-11 nmol/g tissue, Group 3: 95+/-10 nmol/g tissue; Group 1 vs Group 2, p=0.010; Group 1 vs Group 3, p<0.001; Group 2 vs Group 3, p<0.001; Group 1 vs Group 4, p<0.001; Group 2 vs Group 4, p<0.001; Group 3 vs Group 4: p<0.001).

CONCLUSION

Acute skeletal muscle ischemia is a common problem. We are of the opinion that in the early phase of skeletal muscle ischemia, iloprost and pentoxifylline medication may reduce ischemia-reperfusion injury.

摘要

背景

我们在兔模型中研究了伊洛前列素和己酮可可碱对骨骼肌缺血再灌注损伤的影响。

方法

40只新西兰白兔被分为四组。第1组，在腹主动脉阻断形成2小时缺血后再灌注前半小时开始持续输注伊洛前列素，并在4小时再灌注期持续输注。第2组用己酮可可碱治疗，第3组接受生理盐水。第4组为假手术组。评估腓肠肌中的丙二醛水平和水肿评分。

结果

与对照组相比，第1组的水肿评分显著更低（第1组与第3组比较，p = 0.040；第2组与第3组比较，p = 0.145；第1组与第2组比较，p = 0.580）。用药组的丙二醛水平与对照组相比显著更低（第1组：60±11 nmol/g组织，第2组：74±11 nmol/g组织，第3组：95±10 nmol/g组织；第1组与第2组比较，p = 0.010；第1组与第3组比较，p<0.001；第2组与第3组比较，p<0.001；第1组与第4组比较，p<0.001；第2组与第4组比较，p<0.001；第3组与第4组比较：p<0.001）。