Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, Turkey.
Department of Physiology, Dumlupinar University Medical Faculty, Kutahya, Turkey.
J Surg Res. 2014 Mar;187(1):162-8. doi: 10.1016/j.jss.2013.09.031. Epub 2013 Sep 29.
The aim of this study was to investigate the effects of iloprost (IL) on ischemia-reperfusion injury in a rodent model.
Twenty-four Wistar Albino rats were randomized into four groups (n = 6). Laparotomy was performed in all groups under general anesthesia. Only laparotomy was applied in group S (Sham). Ischemia-reperfusion group (group I/R) underwent ischemia and reperfusion performed by clamping and declamping of the infrarenal abdominal aorta for 120 min. The iloprost group (group IL) received intravenous infusion of IL 0.5 ng/kg/min, without I/R. Group I/R + IL received intravenous infusion of IL 0.5 ng/kg/min immediately after 2 h period of ischemia. At the end of the reperfusion period, all rats were killed under anesthesia and skeletal muscle samples of lower extremity were harvested for biochemical and histopathologic analyses.
Tissue levels of endothelial nitric oxide were significantly higher in I/R groups than those in groups S and IL. The heat shock protein 60 levels were higher in group I/R than the other groups. But the heat shock protein 60 levels in group I/R + IL were found to be similar with the groups S and IL. Malondialdehyde levels were significantly higher in group I/R. On the other hand, in group I/R + IL, malondialdehyde levels were higher than those in groups S and IL but lower than those in group I/R. Superoxide dismutase (SOD) enzyme activities were found to be significantly lower in group I/R than the other groups. Also in group I/R/I, the SOD enzyme activities were higher than those in group I/R. But, in group I/R + IL, SOD levels were found to be higher than those in group I/R but lower than those in groups S and IL.
These results indicate that IL has protective effects on I/R injury in skeletal muscle in a rodent model.
本研究旨在探讨前列环素（IL）对动物模型缺血再灌注损伤的影响。
24 只 Wistar 白化大鼠随机分为 4 组（每组 6 只）。所有组均在全麻下进行剖腹手术。仅 S 组（假手术）进行剖腹手术。缺血再灌注组（I/R 组）通过夹闭和松开肾下腹主动脉 120 分钟来进行缺血再灌注。IL 组（IL 组）给予静脉输注 0.5ng/kg/min 的 IL，不进行 I/R。I/R+IL 组在缺血 2 小时后立即给予静脉输注 0.5ng/kg/min 的 IL。再灌注期末，所有大鼠均在麻醉下处死，采集下肢骨骼肌标本进行生化和组织病理学分析。
I/R 组组织内皮型一氧化氮水平明显高于 S 组和 IL 组。I/R 组热休克蛋白 60 水平高于其他组。但 I/R+IL 组热休克蛋白 60 水平与 S 组和 IL 组相似。I/R 组丙二醛水平明显升高。另一方面，在 I/R+IL 组，丙二醛水平高于 S 组和 IL 组，但低于 I/R 组。I/R 组超氧化物歧化酶（SOD）酶活性明显低于其他组。同样在 I/R+IL 组，SOD 酶活性高于 I/R 组。但在 I/R+IL 组，SOD 水平高于 I/R 组，低于 S 组和 IL 组。
这些结果表明，IL 对动物模型骨骼肌缺血再灌注损伤具有保护作用。
