Navarro A, Quiterio M, Benítez O, Uribe F, Cravioto A
División de Microbiologia, Centro de Investigaciones sobre Enfermedades Infecciosas, Cuernavaca, Morelos, México.
Bol Med Hosp Infant Mex. 1991 May;48(5):361-6.
A comparative study between tube agglutination using Spicer-Edwards antisera and a coagglutination method using Staphylococcus aureus Cowan 1 (NCTC 8530) protein A to detect Salmonella flagellar antigens was carried out. A total of 39 Salmonella strains belonging to eight serogroups of the Kauffmann-White scheme were typed by both methods. Each serogroup include monophasic and biphasic strains. Statistical analysis of the results showed that the coagglutination method was more sensitive and specific that the classic tube agglutination method normally used in different laboratories. The use of less antiserum in the coagglutination method to detect Salmonella antigens without loss of specificity or sensitivity represents an important saving for clinical laboratories and for epidemiologic research that require the identification of these antigens.
开展了一项比较研究，比较使用斯派塞 - 爱德华兹抗血清的试管凝集法与使用金黄色葡萄球菌考恩1株（NCTC 8530）蛋白A的协同凝集法检测沙门氏菌鞭毛抗原的效果。采用这两种方法对总共39株属于考夫曼 - 怀特分类系统八个血清群的沙门氏菌菌株进行了分型。每个血清群都包括单相和双相菌株。结果的统计分析表明，协同凝集法比不同实验室通常使用的经典试管凝集法更敏感、更具特异性。在协同凝集法中使用较少的抗血清来检测沙门氏菌抗原，而不损失特异性或敏感性，这对临床实验室以及需要鉴定这些抗原的流行病学研究而言是一项重要的节省。
