Xu Min, Bernát Gábor, Singh Abhay, Mi Hualing, Rögner Matthias, Pakrasi Himadri B, Ogawa Teruo
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Graduate courses of the Chinese Academy of Sciences, Shanghai, 200032, PR China.
Plant Cell Physiol. 2008 Nov;49(11):1672-7. doi: 10.1093/pcp/pcn139. Epub 2008 Sep 10.
A mutant (Delta5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO(2) or HCO(3)(-) and was unable to grow in air with or without glucose. The Delta4 mutant in which BicA is the only active inorganic carbon sequestration system showed low activity of HCO(3)(-) uptake and grew under these conditions but more slowly than the wild-type strain. The Delta5 mutant required 1.7% CO(2) to attain half the maximal growth rate. Electron transport activity of the mutants was strongly inhibited under high light intensities, with the Delta5 mutant more susceptible to high light than the Delta4 mutant. The results implicated the significance of carbon sequestration in dissipating excess light energy.
通过使五个无机碳固定系统失活构建的集胞藻PCC 6803菌株的一个突变体（Delta5）不吸收CO₂或HCO₃⁻，并且在有或没有葡萄糖的空气中均无法生长。其中BicA是唯一活跃的无机碳固定系统的Delta4突变体显示出较低的HCO₃⁻吸收活性，并且在这些条件下能够生长，但比野生型菌株生长得更慢。Delta5突变体需要1.7%的CO₂才能达到最大生长速率的一半。在高光强度下，突变体的电子传递活性受到强烈抑制，Delta5突变体比Delta4突变体对高光更敏感。结果表明碳固定在消散过量光能方面的重要性。
