低温甲基丙烯酸甲酯树脂包埋山羊组织的免疫组织化学分析

Immunohistochemical analysis of low-temperature methylmethacrylate resin-embedded goat tissues.

作者信息

Vertenten G, Vlaminck L, Ducatelle R, Lippens E, Cornelissen M, Gasthuys F

机构信息

Department of Surgery and anaesthesiology of domestic animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.

出版信息

Anat Histol Embryol. 2008 Dec;37(6):452-7. doi: 10.1111/j.1439-0264.2008.00881.x. Epub 2008 Sep 10.

DOI:10.1111/j.1439-0264.2008.00881.x

PMID:18786161

Abstract

Goats are frequently used as a suitable animal model for tissue engineering. Immunohistochemistry can be helpful in improving the understanding and evaluation of the in vivo tissue responses at a molecular level. Several commercially available antibodies (KI67, vimentin, CD31, core-binding factor alpha-1, osteocalcin, alkaline phosphatase, MAC387, CD3, CD20, CD20cy, CD79 and CD45) were evaluated on Technovit 9100 New embedded goat tissues. Only vimentin, osteocalcin, MAC387 and CD3 revealed positive staining. These antibodies can be routinely used to evaluate goat tissues at molecular level. The use and development of alternative antibodies might further supplement and complete the possibilities for immunohistochemical analysis of goat tissue samples.

摘要

山羊经常被用作组织工程的合适动物模型。免疫组织化学有助于在分子水平上增进对体内组织反应的理解和评估。在Technovit 9100新包埋的山羊组织上评估了几种市售抗体（KI67、波形蛋白、CD31、核心结合因子α-1、骨钙素、碱性磷酸酶、MAC387、CD3、CD20、CD20cy、CD79和CD45）。只有波形蛋白、骨钙素、MAC387和CD3显示出阳性染色。这些抗体可常规用于在分子水平上评估山羊组织。替代抗体的使用和开发可能会进一步补充并完善山羊组织样本免疫组织化学分析的可能性。