巴西利什曼原虫（维阿尼亚种）：用于聚合酶链反应鉴定的新型引物
Leishmania (Viannia) braziliensis: New primers for identification using polymerase chain reaction.
作者信息
Marcussi V M, Marcussi L M, Barbosa-Tessmann I P, Lonardoni M V C, Silveira T G V
机构信息
Master program in Clinical Analyses, Universidade Estadual de Maringá, Maringá, PR, Brazil.
出版信息
Exp Parasitol. 2008 Dec;120(4):300-5. doi: 10.1016/j.exppara.2008.08.005. Epub 2008 Aug 26.
The objective of this study was to develop specific primers for Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various L. (V.) braziliensis serodemes and various Leishmania species and also using Trypanosoma cruzi. A specific fragment of 536bp was detected from 50ng of DNA in a crude extract derived from L. (V.) braziliensis. The DNA fragment was not detected when DNA from other Leishmania species or from T. cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify L. (V.) braziliensis, they could be considered for use in L. (V.) braziliensis diagnosis and epidemiological studies.
本研究的目的是开发用于通过聚合酶链反应（PCR）鉴定巴西利什曼原虫（维安尼亚亚属）的特异性引物。使用各种巴西利什曼原虫（维安尼亚亚属）血清型、各种利什曼原虫物种以及克氏锥虫对设计的引物（LBF1和LBR1）进行敏感性和特异性评估。从巴西利什曼原虫（维安尼亚亚属）粗提物中50ng DNA检测到一条536bp的特异性片段。当使用来自其他利什曼原虫物种或克氏锥虫的DNA作为PCR模板时，未检测到该DNA片段。此外，用皮肤利什曼病的DNA进行检测时，设计的引物和反应给出了阳性结果。考虑到引物LBF1和LBR1可以特异性鉴定巴西利什曼原虫（维安尼亚亚属），它们可用于巴西利什曼原虫（维安尼亚亚属）的诊断和流行病学研究。
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