Bhatia Kanchan, Ahmad Firoz, Rashid Hina, Raisuddin Sheikh
Department of Medical Elementology and Toxicology, Hamdard University, New Delhi 110 062, India.
Food Chem Toxicol. 2008 Nov;46(11):3368-74. doi: 10.1016/j.fct.2008.08.011. Epub 2008 Aug 22.
S-Allylcysteine (SAC), an organosulfur compound of aged garlic extract (AGE) regulates the thiol status of the cell and scavenges free radicals. Depletion of thiols along with free radical generation has been implicated in cyclophosphamide (CP)-induced urotoxicity. We studied modulatory effect of SAC on CP-induced urotoxicity in mice focusing on hemorrhagic cystitis (HC). SAC (150 and 300 mg kg(-1)) was administered in CP treated animals (200 mg kg(-1)) and bladder was observed for histological and biochemical changes. CP treatment caused a marked increase in the lumen exudates, edema, vasodilation and HC in lamina propia in the bladder. These changes were accompanied by increase in lipid peroxidation (LPO), and decrease in reduced glutathione (GSH) and activities of antioxidant enzymes. SAC not only showed protection in tissue histology but also improved the decreased activities of antioxidant enzymes. SAC treatment also reduced LPO and increased GSH levels. Although SAC treatment did not ensure full recovery, the marked improvement in histology and antioxidants of bladder suggests that it has a significant modulatory effect on CP-induced urotoxicity. Since decrease in antioxidant level is the major cause of CP urotoxicity, the protective effect of SAC deserves its further exploration involving laboratory and clinical investigations.
S-烯丙基半胱氨酸（SAC）是一种陈年大蒜提取物（AGE）中的有机硫化合物，可调节细胞的硫醇状态并清除自由基。硫醇的消耗以及自由基的产生与环磷酰胺（CP）诱导的尿毒性有关。我们研究了SAC对CP诱导的小鼠尿毒性的调节作用，重点关注出血性膀胱炎（HC）。在接受CP治疗的动物（200mg kg-1）中给予SAC（150和300mg kg-1），观察膀胱的组织学和生化变化。CP治疗导致膀胱固有层管腔渗出物、水肿、血管舒张和HC显著增加。这些变化伴随着脂质过氧化（LPO）增加、还原型谷胱甘肽（GSH）减少以及抗氧化酶活性降低。SAC不仅在组织组织学上显示出保护作用，还改善了抗氧化酶活性的降低。SAC治疗还降低了LPO并提高了GSH水平。虽然SAC治疗不能确保完全恢复，但膀胱组织学和抗氧化剂的显著改善表明它对CP诱导的尿毒性具有显著的调节作用。由于抗氧化剂水平降低是CP尿毒性的主要原因，SAC的保护作用值得进一步进行实验室和临床研究探索。
