原代培养气道杯状细胞中P2嘌呤受体对粘蛋白释放的调节

P2 purinoceptor regulation of mucin release by airway goblet cells in primary culture.

Kim K C, Lee B C

Department of Pharmacology and Toxicology, School of Pharmacy, University of Maryland, Baltimore 21201.

Br J Pharmacol. 1991 May;103(1):1053-6. doi: 10.1111/j.1476-5381.1991.tb12299.x.

The effects of adenine analogues on mucin release by airway goblet cells have been examined in a hamster primary tracheal epithelial cell culture. 2. Adenosine, a P1 receptor agonist, had no effect on mucin release even at 2 mM, while ATP, a P2 receptor agonist, stimulated mucin release in a dose-dependent fashion with an apparent EC50 of 20 microM. The relative potency order among adenine nucleotides was ATP greater than ADP greater than AMP = adenosine. 3. ATP gamma S, a non-hydrolyzable analogue of ATP, was equipotent with ATP in stimulating mucin release. The potency order among some ATP analogues was ATP greater than 2-methylthio ATP greater than alpha, beta-methylene ATP greater than beta, gamma-methylene ATP. Reactive blue 2, a putative P2 gamma-purinoceptor antagonist, did not block the ATP-induced mucin release. 4. The present results indicate that mucin release by airway goblet cells is stimulated by extracellular ATP via P2 receptor-mediated mechanism. We suggest that this mechanism may be important in the physiological regulation of airway goblet cell mucin release in vivo.

已在仓鼠原代气管上皮细胞培养物中研究了腺嘌呤类似物对气道杯状细胞黏蛋白释放的影响。2. 腺苷是一种P1受体激动剂，即使在2 mM时对黏蛋白释放也无影响，而ATP是一种P2受体激动剂，以剂量依赖方式刺激黏蛋白释放，表观EC50为20 microM。腺嘌呤核苷酸之间的相对效力顺序为ATP大于ADP大于AMP = 腺苷。3. ATPγS是一种不可水解的ATP类似物，在刺激黏蛋白释放方面与ATP等效。一些ATP类似物之间的效力顺序为ATP大于2-甲硫基ATP大于α,β-亚甲基ATP大于β,γ-亚甲基ATP。活性蓝2是一种假定的P2γ嘌呤受体拮抗剂，不能阻断ATP诱导的黏蛋白释放。4. 目前的结果表明，气道杯状细胞的黏蛋白释放是由细胞外ATP通过P2受体介导的机制刺激的。我们认为这种机制可能在体内气道杯状细胞黏蛋白释放的生理调节中很重要。

P2 purinoceptor regulation of mucin release by airway goblet cells in primary culture.