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铜绿假单胞菌的蛋白酶可引起气管上皮释放黏蛋白。

Proteinases of Pseudomonas aeruginosa evoke mucin release by tracheal epithelium.

作者信息

Klinger J D, Tandler B, Liedtke C M, Boat T F

出版信息

J Clin Invest. 1984 Nov;74(5):1669-78. doi: 10.1172/JCI111583.

DOI:10.1172/JCI111583
PMID:6568227
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC425344/
Abstract

We have determined the potential of exoproducts from pathogenic bacteria to stimulate the release of high molecular weight mucins from goblet cells of airway epithelium in a rabbit tracheal explant system. Culture supernatants from proteolytic strains of Pseudomonas aeruginosa and Serratia marcescens, but not supernatants from a number of non-proteolytic strains, released mucins from goblet cells. Highly purified elastase and alkaline proteinase from P. aeruginosa stimulated goblet cell mucin release in a dose-dependent fashion. Lipopolysaccharide, exotoxin A, and alginate of P. aeruginosa did not possess mucin release properties. Proteolytic activity was required for mucin release by P. aeruginosa elastase, but such release in goblet cells was not mediated by cyclic AMP. Morphologic studies suggested rapid release of mucins from goblet cells was response to elastase by a process resembling apocrine secretion. Several nonbacterial proteinases mimicked the effect of Pseudomonas proteases. These studies provide support for the hypothesis that bacterial and other play a role in the pathogenesis of mucus hypersecretion in acute and chronic lung infections.

摘要

我们已经在兔气管外植体系统中确定了病原菌外产物刺激气道上皮杯状细胞释放高分子量粘蛋白的潜力。铜绿假单胞菌和粘质沙雷氏菌的蛋白水解菌株的培养上清液可使杯状细胞释放粘蛋白，而许多非蛋白水解菌株的上清液则不能。铜绿假单胞菌高度纯化的弹性蛋白酶和碱性蛋白酶以剂量依赖方式刺激杯状细胞粘蛋白释放。铜绿假单胞菌的脂多糖、外毒素A和藻酸盐不具有粘蛋白释放特性。铜绿假单胞菌弹性蛋白酶释放粘蛋白需要蛋白水解活性，但杯状细胞中的这种释放不是由环磷酸腺苷介导的。形态学研究表明，杯状细胞中粘蛋白的快速释放是对弹性蛋白酶的反应，其过程类似于顶浆分泌。几种非细菌性蛋白酶模拟了假单胞菌蛋白酶的作用。这些研究为细菌及其他因素在急慢性肺部感染中粘液分泌过多的发病机制中起作用这一假说提供了支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/9d0d31d58fc5/jcinvest00137-0128-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/466261968d8a/jcinvest00137-0127-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/6c1e3043b994/jcinvest00137-0127-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/04fc91f6dfb4/jcinvest00137-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/cc260eddec7b/jcinvest00137-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/9d0d31d58fc5/jcinvest00137-0128-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/466261968d8a/jcinvest00137-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/b7b4dee3030f/jcinvest00137-0127-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/6c1e3043b994/jcinvest00137-0127-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/04fc91f6dfb4/jcinvest00137-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/cc260eddec7b/jcinvest00137-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef25/425344/9d0d31d58fc5/jcinvest00137-0128-c.jpg

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本文引用的文献

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