Eberhardson Michael, Lindberg Kenneth, Karlsson Mats, Karlén Per, Winqvist Ola, Thörn Magnus
aDepartment of Clinical Science and Education, Sodersjukhuset, Karolinska Institutet, Stockholm, Sweden.
Eur J Gastroenterol Hepatol. 2008 Oct;20(10):985-8. doi: 10.1097/MEG.0b013e3282fbf5f5.
Mesenteric lymph nodes may play a crucial role in the pathogenesis of human inflammatory bowel disease. We have used the sentinel node technique to analyze mesenteric lymph nodes draining inflammatory lesions sentinel nodes and corresponding site of inflamed bowel in patients with ulcerative colitis or Crohn's disease.
Thirty-two patients undergoing surgical treatment of inflammatory bowel disease were included. Sentinel nodes were identified intraoperatively. The T cells were harvested from the mesenteric lymph nodes and characterized by flow cytometry.
The distribution of CD4CD62L (homing-marked) and CD4CD69 (activated) T cells was studied in mesenteric lymph nodes draining inflammation, nodes draining normal intestine, blood, and mucosa. The turnover of T cells was markedly increased in the lymph nodes connected to inflammatory segments. The immunologic activity of the sentinel lymph nodes correlated with the degree of intestinal inflammation.
Mesenteric sentinel nodes provide important information about locoregional immunology and pathogenesis in inflammatory bowel disease.
肠系膜淋巴结可能在人类炎症性肠病的发病机制中起关键作用。我们采用前哨淋巴结技术分析溃疡性结肠炎或克罗恩病患者引流炎症病变的肠系膜淋巴结（前哨淋巴结）及相应炎症肠段部位。
纳入32例接受炎症性肠病手术治疗的患者。术中识别前哨淋巴结。从肠系膜淋巴结中采集T细胞，并用流式细胞术进行特征分析。
研究了引流炎症的肠系膜淋巴结、引流正常肠段的淋巴结、血液及黏膜中CD4CD62L（归巢标记）和CD4CD69（活化）T细胞的分布。与炎症肠段相连的淋巴结中T细胞周转率显著增加。前哨淋巴结的免疫活性与肠道炎症程度相关。
肠系膜前哨淋巴结提供了有关炎症性肠病局部区域免疫学和发病机制的重要信息。
