Smiderle Fhernanda R, Olsen Lorena M, Carbonero Elaine R, Baggio Cristiane H, Freitas Cristina S, Marcon Rodrigo, Santos Adair R S, Gorin Philip A J, Iacomini Marcello
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
Eur J Pharmacol. 2008 Nov 12;597(1-3):86-91. doi: 10.1016/j.ejphar.2008.08.028. Epub 2008 Aug 29.
A glucan was extracted with hot water from the basidiomycete Pleurotus pulmonarius and shown to have a (1-->3)-linked beta-D-glucopyranosyl main-chain substituted at O-6 of every third unit by single beta-D-glucopyranosyl non-reducing end units. This was shown by mono- and bidimensional nuclear magnetic resonance (NMR) spectroscopy, methylation analysis, and a controlled Smith degradation. The glucan was tested for its effects on the acetic acid-induced writhing reaction in mice, a typical model for quantifying inflammatory pain. It caused a marked and dose-dependent anti-inflammatory response, demonstrated by the inhibition of leukocyte migration to injured tissues (82 +/- 6%) with an ID50 of 1.19 (0.74-1.92) mg/kg. Furthermore, animals previously treated with the glucan (3 mg/kg i.p.), showed a reduction of 85 +/- 5% of writhes, after receiving the acetic acid injection. Furthermore, in the formalin test, the glucan (3-30 mg/kg, i.p.) also caused significant inhibition of both the early (neurogenic pain) and the late phases (inflammatory pain) of formalin-induced licking. However, it was more potent and effective in relation to the late phase of the formalin test, with mean ID(50) values for the neurogenic and the inflammatory phases of > 30 and 12.9 (6.7-24.6) mg/kg and the inhibitions observed were 43 +/- 5% and 96 +/- 4%, respectively. These data showed that the glucan had potent anti-inflammatory and analgesic (antinociceptive) activities, possibly by the inhibition of pro-inflammatory cytokines.
从担子菌侧耳中用热水提取了一种葡聚糖,结果表明其具有以(1→3)连接的β -D-吡喃葡萄糖基主链,每隔三个单元的O-6位被单个β -D-吡喃葡萄糖基非还原末端单元取代。这通过一维和二维核磁共振(NMR)光谱、甲基化分析以及可控的史密斯降解得以证明。对该葡聚糖进行了测试,观察其对小鼠醋酸诱导扭体反应的影响,这是一种用于量化炎性疼痛的典型模型。它引起了显著的剂量依赖性抗炎反应,表现为对白细胞向损伤组织迁移的抑制作用(82±6%),半数抑制剂量(ID50)为1.19(0.74 - 1.92)mg/kg。此外,预先经该葡聚糖(3 mg/kg腹腔注射)处理的动物,在注射醋酸后,扭体次数减少了85±5%。此外,在福尔马林试验中,该葡聚糖(3 - 30 mg/kg,腹腔注射)也显著抑制了福尔马林诱导舔舐行为的早期(神经源性疼痛)和晚期(炎性疼痛)阶段。然而,它在福尔马林试验晚期更有效,神经源性和炎性阶段的平均ID50值分别>30和12.9(6.7 - 24.6)mg/kg,观察到的抑制率分别为43±5%和96±4%。这些数据表明,该葡聚糖具有强大的抗炎和镇痛(抗伤害感受)活性,可能是通过抑制促炎细胞因子实现的。