Taha Taher M, Kanao Tadayoshi, Takeuchi Fumiaki, Sugio Tsuyoshi
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Appl Environ Microbiol. 2008 Nov;74(21):6808-10. doi: 10.1128/AEM.00787-08. Epub 2008 Sep 12.
The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.
来自以硫为生长底物的氧化亚铁硫杆菌ATCC 23270细胞的铁氧化系统在体外被重建。纯化的锈铁蛋白、细胞色素c和aa(3)型细胞色素氧化酶对于重建至关重要。重建系统的铁氧化活性比从中纯化出这些组分的细胞提取物的铁氧化活性高3.3倍。