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YB-1通过与微管蛋白和微管相互作用在体外促进微管组装。

YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.

作者信息

Chernov Konstantin G, Mechulam Alain, Popova Nadezhda V, Pastre David, Nadezhdina Elena S, Skabkina Olga V, Shanina Nina A, Vasiliev Victor D, Tarrade Anne, Melki Judith, Joshi Vandana, Baconnais Sonia, Toma Flavio, Ovchinnikov Lev P, Curmi Patrick A

机构信息

Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, INSERM/UEVE U829 Evry, 91025 France.

出版信息

BMC Biochem. 2008 Sep 15;9:23. doi: 10.1186/1471-2091-9-23.

DOI:10.1186/1471-2091-9-23
PMID:18793384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2557009/
Abstract

BACKGROUND

YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.

RESULTS

We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.

CONCLUSION

These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation.

摘要

背景

YB - 1是真核细胞中基因表达的主要调节因子。除了在转录中的作用外，YB - 1在mRNA的翻译和稳定中也起着关键作用。

结果

我们在此表明，YB - 1与微管蛋白和微管相互作用，并在体外刺激微管组装。通过电子显微镜和原子力显微镜进行的高分辨率成像显示，在YB - 1存在下组装的微管呈现出正常的单壁超微结构，并表明YB - 1很可能覆盖在微管外壁上。此外，我们发现YB - 1还促进微管相关蛋白 - 微管蛋白和枯草杆菌蛋白酶处理的微管蛋白的组装。最后，我们证明微管蛋白会干扰RNA:YB - 1复合物。

结论

这些结果表明，YB - 1可能在体内调节微管组装，并且其与微管蛋白的相互作用可能有助于控制mRNA翻译。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/8f2b3d7a1bde/1471-2091-9-23-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/fc911efd0de8/1471-2091-9-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/398258de8a73/1471-2091-9-23-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/e05f3f863e53/1471-2091-9-23-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/427f18c44205/1471-2091-9-23-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/17a1999479a3/1471-2091-9-23-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/4b68adc41648/1471-2091-9-23-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/76fd1a1de87e/1471-2091-9-23-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/adecb77a4a1b/1471-2091-9-23-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/553ddbb7f461/1471-2091-9-23-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/8f2b3d7a1bde/1471-2091-9-23-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/fc911efd0de8/1471-2091-9-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/398258de8a73/1471-2091-9-23-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/e05f3f863e53/1471-2091-9-23-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/427f18c44205/1471-2091-9-23-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/17a1999479a3/1471-2091-9-23-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/4b68adc41648/1471-2091-9-23-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/76fd1a1de87e/1471-2091-9-23-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/adecb77a4a1b/1471-2091-9-23-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/553ddbb7f461/1471-2091-9-23-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df0e/2557009/8f2b3d7a1bde/1471-2091-9-23-10.jpg

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