Ponzoni Maurilio, Ferreri Andrés J M, Guidoboni Massimo, Lettini Antonia A, Cangi Maria Giulia, Pasini Elisa, Sacchi Luciano, Pecciarini Lorenza, Grassi Stefano, Dal Cin Elena, Stefano Rosalba, Magnino Simone, Dolcetti Riccardo, Doglioni Claudio
Pathology Unit, San Raffaele Scientific Institute, Milano, Italy.
Clin Cancer Res. 2008 Sep 15;14(18):5794-800. doi: 10.1158/1078-0432.CCR-08-0676.
Chlamydia psittaci (Cp) has been associated to ocular adnexal lymphomas (OAL) with variable geographic distribution. Herein, we used multiple Chlamydia detection tools to identify Cp elementary bodies-containing cell and to assess Cp prevalence in both nodal and extranodal lymphomas.
TETR-PCR, immunohistochemistry, immunofluorescence, electron microscopy, and laser-capture microdissection were done in 35 OALs to define their effect in Chlamydia detection and, moreover, to identify the Cp cellular carrier. Cp prevalence was screened by TETR-PCR in 205 extraorbital lymphomas and 135 nonneoplastic controls.
Twenty-six (74%) OALs were associated with Cp infection: immunohistochemistry, immunofluorescence, and laser-capture microdissection-assisted PCR showed that monocytes/macrophages were the Cp carriers; electron microscopy showed the presence of intact Cp elementary bodies into these cells. Immunohistochemistry and TETR-PCR showed a 70% concordance rate (P = 0.001). Cp DNA was equally prevalent in non-OAL, nodal, and extranodal lymphomas: among the latter, it was more common in diffuse large B-cell lymphomas of the skin (P = 0.03) and Waldeyer's ring.
This multiparametric approach shows, for the first time, that monocytes/macrophages are the carriers of Cp, Cp seems preferentially associated with lymphomas arising in organs primarily exposed to antigens. The clinical implications of these findings deserve to be prospectively investigated.
鹦鹉热衣原体（Cp）与眼部附属器淋巴瘤（OAL）有关，其地理分布各异。在此，我们使用多种衣原体检测工具来识别含有Cp原体的细胞，并评估Cp在淋巴结和结外淋巴瘤中的流行情况。
对35例OAL进行了四引物PCR、免疫组织化学、免疫荧光、电子显微镜和激光捕获显微切割，以确定它们在衣原体检测中的作用，并进一步识别Cp的细胞载体。通过四引物PCR对205例眶外淋巴瘤和135例非肿瘤对照进行Cp流行情况筛查。
26例（74%）OAL与Cp感染有关：免疫组织化学、免疫荧光和激光捕获显微切割辅助PCR显示单核细胞/巨噬细胞是Cp的载体；电子显微镜显示这些细胞内存在完整的Cp原体。免疫组织化学和四引物PCR的符合率为70%（P = 0.001）。Cp DNA在非OAL、淋巴结和结外淋巴瘤中的流行情况相同：在后者中，它在皮肤弥漫性大B细胞淋巴瘤（P = 0.03）和瓦尔代尔环中更为常见。
这种多参数方法首次表明单核细胞/巨噬细胞是Cp的载体，Cp似乎优先与主要暴露于抗原的器官中发生的淋巴瘤相关。这些发现的临床意义值得进行前瞻性研究。
