Koechlin N, Pisam M, Poujeol P, Tauc M, Rambourg A
Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.
Eur J Cell Biol. 1991 Apr;54(2):224-36.
The evolution of a primary culture of kidney proximal convoluted tubule (PCT) cells was followed step by step from the plating time of an isolated tubule to the 39th day of culture. During the first 48 h, the structural remodeling of PCT, leading to the formation of a cell monolayer without cell division, is accompanied by intracytoplasmic changes indicating cell dedifferentiation. Numerous autophagic vacuoles are observed inside the cells, and the ultrastructural features characteristic of in situ PCT cells are progressively lost. Despite these drastic modifications, cell polarity, as observed by immunocytochemical detection of the leucine aminopeptidase, remains unaltered. Starting at 48 h, the peripheral cells divide, and the culture proliferates in a centrifugal direction while newly formed cells differentiate. From 6 days onwards, glycogen granules, never encountered in in situ PCT cells, appear in cultured cells and progressively accumulate. At the optimal stage of the culture (12-17 days old), cells somewhat resemble PCT cells, but their apical brush borders remain rudimentary, and basal cytoplasmic interdigitations surrounding densely packed mitochondria are poorly developed. Subsequently, the cells become overloaded with glycogen and lipid inclusions and resemble degenerating cells.
从分离的肾小管接种时间到培养的第39天，对肾近端小管（PCT）细胞原代培养的演变过程进行了逐步跟踪。在最初的48小时内，PCT的结构重塑导致形成无细胞分裂的细胞单层，同时伴有表明细胞去分化的胞浆内变化。细胞内观察到大量自噬泡，原位PCT细胞的超微结构特征逐渐丧失。尽管有这些剧烈变化，但通过亮氨酸氨肽酶免疫细胞化学检测观察到的细胞极性保持不变。从48小时开始，周边细胞开始分裂，培养物沿离心方向增殖，同时新形成的细胞发生分化。从第6天起，原位PCT细胞中从未见过的糖原颗粒出现在培养细胞中并逐渐积累。在培养的最佳阶段（12 - 17天龄），细胞 somewhat resemble PCT细胞，但其顶端刷状缘仍不发达，围绕密集排列线粒体的基底胞质交错也发育不良。随后，细胞中糖原和脂质包涵体过载，类似于退化细胞。 （注：原文中“somewhat resemble”这里的“somewhat”不太好准确翻译，直接保留英文是一种处理方式，也可勉强译为“有点类似”等，但整体译文可能稍显生硬，因为原文表述稍显奇怪。）
