Hård K, Spronk B A, Hokke C H, Kamerling J P, Vliegenthart J F
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
FEBS Lett. 1991 Aug 5;287(1-2):108-12. doi: 10.1016/0014-5793(91)80027-z.
Three glycoprotein N-glycans, namely, a disialylated diantennary carbohydrate chain linked to Asn, a monosialylated, fucosylated diantennary glycopeptide with bisecting N-acetylglucosamine, and a tetrasialylated, fucosylated tetra-antennary oligosaccharide, have been investigated by two-dimensional NOE and HOHAHA spectroscopy in 1H2O as solvent. The amide protons of all N-acetylglucosamine and sialic acid residues could readily be assigned. The large chemical-shift dispersion of the amide resonances of the N-acetylglucosamine residues, allowed the unambiguous assignment of every N-acetyl methyl signal, via strong NOEs. Subspectra could be obtained of all N-acetylglucosamine residues in HOHAHA spectra. These results have as main implication that several biologically important large glycans will now [corrected] become amenable for conformational studies by multidimensional NMR in 1H2O solution.
三种糖蛋白N-聚糖，即与天冬酰胺相连的二唾液酸化二天线碳水化合物链、具有平分型N-乙酰葡糖胺的单唾液酸化、岩藻糖基化二天线糖肽以及四唾液酸化、岩藻糖基化四天线寡糖，已在以1H2O为溶剂的条件下通过二维NOE和HOHAHA光谱进行了研究。所有N-乙酰葡糖胺和唾液酸残基的酰胺质子都能很容易地被归属。N-乙酰葡糖胺残基酰胺共振的大化学位移分散，使得通过强NOE能明确归属每个N-乙酰甲基信号。在HOHAHA光谱中可以获得所有N-乙酰葡糖胺残基的子光谱。这些结果的主要意义在于，现在几种具有生物学重要性的大聚糖将适用于在1H2O溶液中通过多维NMR进行构象研究。
