Lin Pin-Pin, Yang Ming-Hui, Liao Pao-Chi, Wu Hsin-Yi, Chang Louis W, Tsai Hui-Ti, Tyan Yu-Chang
Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Taiwan.
Biochem Biophys Res Commun. 2008 Nov 21;376(3):519-24. doi: 10.1016/j.bbrc.2008.09.020. Epub 2008 Sep 15.
Trans, trans-2,4-decadienal (tt-DDE), a specific type of dienaldehyde, is abundant in heated oils or cooking oil fumes. Ingestion of heated oils and exposure to cooking oil fumes has been suggested to have a great health impact in a variety of organs, including the lungs. Previous studies have demonstrated that acute exposures to high doses of tt-DDE have induced oxidative stress, genotoxicity, and cytotoxicity in human lung cells. The objective in utilizing proteomic techniques of this study was to identify protein biomarkers associated with tt-DDE-induced oxidative stress and cytotoxicity in human bronchial epithelial cells BEAS-2B. Experimental results suggested that DJ-1 and cofilin proteins were protein biomarkers for tt-DDE-induced cytotoxicity and oxidative stress in lung cells. DJ-1 was especially an early biomarker for tt-DDE exposure.
反，反-2,4-癸二烯醛（tt-DDE）是一种特定类型的二烯醛，在加热油或烹饪油烟中含量丰富。摄入加热油和接触烹饪油烟已被认为会对包括肺部在内的多种器官产生重大健康影响。先前的研究表明，急性高剂量暴露于tt-DDE会在人肺细胞中诱导氧化应激、遗传毒性和细胞毒性。本研究利用蛋白质组学技术的目的是鉴定与tt-DDE诱导的人支气管上皮细胞BEAS-2B氧化应激和细胞毒性相关的蛋白质生物标志物。实验结果表明，DJ-1和丝切蛋白是tt-DDE诱导的肺细胞细胞毒性和氧化应激的蛋白质生物标志物。DJ-1尤其是tt-DDE暴露的早期生物标志物。
