一种用于链霉菌属的硫链丝菌素诱导型表达载体
A thiostrepton-inducible expression vector for use in Streptomyces spp.
作者信息
Kuhstoss S, Rao R N
机构信息
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285.
出版信息
Gene. 1991 Jul 15;103(1):97-9. doi: 10.1016/0378-1119(91)90398-u.
PMID:1879704
Abstract
A shuttle expression vector containing the thiostrepton-inducible Streptomyces lividans promoter, ptipA, and the origin of transfer from plasmid RP4 was constructed. Cassettes containing a promoterless xylE gene upstream from a hyg gene were used to demonstrate thiostrepton-inducible expression from ptipA in both S. lividans and Streptomyces ambofaciens, ptipA was estimated to be induced 60-fold or more in Streptomyces ambofaciens.
摘要
构建了一个穿梭表达载体，其包含硫链丝菌素诱导型的淡紫灰链霉菌启动子ptipA和来自质粒RP4的转移起始位点。含有位于潮霉素基因上游的无启动子木糖异构酶基因（xylE）的盒式结构用于证明ptipA在淡紫灰链霉菌和栖土曲霉中均具有硫链丝菌素诱导型表达，据估计ptipA在栖土曲霉中可被诱导60倍或更高倍数。
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