Tan Ji-chun, Li Yang, Qu Wen-yu, Liu Li-ying, Jiang Li, Sun Kai-lai
Department of Medical Genetics, China Medical University, Shenyang, China.
Neuroreport. 2008 Oct 8;19(15):1451-5. doi: 10.1097/WNR.0b013e32830e4c35.
We established a human embryonic stem cell line derived from frozen human embryos of Chinese origin. The cell line expressed the pluripotent markers SSEA-4, TRA-1-60, TRA-1-81, Oct-4, and alkaline phosphatase. The pluripotency of the cell line was also demonstrated in vivo by teratoma formation in severe combined immunodeficiency mice. The embryonic stem cells formed embryoid bodies after culturing in suspension for 7 days. The embryoid bodies were transferred to an adherent culture system in serum-free medium. The differentiating cells derived from the embryoid bodies expressed Nestin and Sox2, markers of neural progenitor cells. After the induction of cyclic AMP for 7 days, the neural progenitor cells had differentiated into neurons and glial cells.
我们建立了一个源自中国冷冻人类胚胎的人胚胎干细胞系。该细胞系表达多能性标志物SSEA-4、TRA-1-60、TRA-1-81、Oct-4和碱性磷酸酶。该细胞系的多能性还通过在严重联合免疫缺陷小鼠体内形成畸胎瘤得到了体内验证。胚胎干细胞在悬浮培养7天后形成胚状体。将胚状体转移至无血清培养基中的贴壁培养系统。源自胚状体的分化细胞表达神经祖细胞标志物Nestin和Sox2。在诱导环磷酸腺苷7天后，神经祖细胞分化为神经元和神经胶质细胞。
