Charrua Ana, Reguenga Carlos, Paule Cleoper C, Nagy Istvan, Cruz Francisco, Avelino António
Institute of Histology and Embriology, University of Porto, Porto, Portugal.
Neuroreport. 2008 Oct 8;19(15):1469-72. doi: 10.1097/WNR.0b013e32830f1e73.
The recently identified splice variant of the transient receptor vanilloid type 1 (TRPV1) molecule, TRPV1b, produces a negative-dominant effect on the responsiveness of the TRPV1 channel, which is increased by peripheral inflammatory processes. Here, we studied, using real-time polymerase chain reaction, whether cyclophosphamide injection-evoked cystitis is associated with altered TRPV1/TRPV1b expression in the L5-L6 dorsal root ganglia, which innervate the urinary bladder. We found that while TRPV1 mRNA expression was unchanged, the amount of TRPV1b transcript was significantly reduced in L5-L6 dorsal root ganglia during cystitis. These data indicate that peripheral inflammatory events induce changes in TRPV1b expression in primary sensory neurons, which may result in increased responsiveness of the TRPV1 channel.
最近发现的瞬时受体香草酸亚型1（TRPV1）分子的剪接变体TRPV1b，对TRPV1通道的反应性产生负显性效应，而外周炎症过程会增强该通道的反应性。在此，我们运用实时聚合酶链反应，研究了注射环磷酰胺诱发的膀胱炎是否与支配膀胱的L5-L6背根神经节中TRPV1/TRPV1b表达的改变有关。我们发现，虽然TRPV1 mRNA表达未变，但膀胱炎期间L5-L6背根神经节中TRPV1b转录本的量显著减少。这些数据表明，外周炎症事件会诱导初级感觉神经元中TRPV1b表达的变化，这可能导致TRPV1通道反应性增加。
