Kravtsov E G, Gukasian I A, Dolgikh M S, Ermolaev A V, Spivak S M
Gig Tr Prof Zabol. 1991(3):33-4.
The membrane filtration technique and column gel chromatography+ (with sepharose 6B or sephacryl S-200) were used to receive fractions of KJ Candida maltosa, strain VSB 899, which were studied with highly effective liquid chromatography, immunochemical reactions and intracutaneous allergotesting on guinea-pigs sensibilized with VSB 899 live cultures. In the whole KJ strain VSB 899 concentrate, 8 components were identified, six of which had molecular masses from 350,000 to 18,000 and had no analogue in the surface antigen preparation (SAP) from the same strain of yeast-like fungi. It was shown that the SAP-related antigen determinants were present in predominantly polysaccharide-containing fractions. In intracutaneous allergotesting, chromatographic fractions with molecular masses of 50,000 and 18,000-35,000 were most manifesting. It was supposed that still further purification of the allergen will increase its specificity.
采用膜过滤技术和柱凝胶色谱法（使用琼脂糖6B或Sephacryl S - 200）对麦芽糖假丝酵母KJ菌株VSB 899进行分级分离，并用高效液相色谱、免疫化学反应以及对用VSB 899活培养物致敏的豚鼠进行皮内变应原试验对其进行研究。在整个KJ菌株VSB 899浓缩物中，鉴定出8种成分，其中6种的分子量在350,000至18,000之间，并且在来自同一株类酵母真菌的表面抗原制剂（SAP）中没有类似物。结果表明，与SAP相关的抗原决定簇主要存在于含多糖的级分中。在皮内变应原试验中，分子量为50,000以及18,000 - 35,000的色谱级分表现最为明显。据推测，进一步纯化变应原将提高其特异性。
