Glabowski Wojciech, Wiszniewska Barbara, Kurzawa Rafal
Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland.
J Assist Reprod Genet. 2008 Aug;25(8):395-402. doi: 10.1007/s10815-008-9248-8. Epub 2008 Sep 17.
To examine the effect of stem cell factor (SCF) to embryos exposed to detrimental factors.
Mice embryos cultured in control medium or Exp.1. with FasL or FasL+SCF Exp.2. with hydrogen peroxide (HP) or HP+SCF; Exp.3. frozen-thawed and cultured with or without SCF. Immunohistochemistry for Fas and c-kit receptors was performed in blastocysts. Blastocyst rates, total numbers of blastocyst cells (TB) and inner cell mass cell counts (ICM) were determined.
Immunohistochemical studies revealed expression of both Fas and c-kit in blastocyst cells. Exp.1. Significantly more blastocysts were found in control when compared to FasL group and to FasL+SCF group. TB and ICM counts in control and FasL+SCF group were significantly higher comparing to FasL group. Exp.2. We found significant differences between three groups in all three evaluated parameters. The highest blastocyst rates, TB and ICM counts were found in control, lower in HP+SCF group and the worst in HP group. Exp.3. No significant differences in TB and ICM counts were found. More embryos formed blastocyst in control than in two cryopreserved groups. Blastocyst rates did not differ between two cryopreserved groups.
SCF may improve culture of embryos exposed to unfavorable milieu.
研究干细胞因子（SCF）对暴露于有害因素的胚胎的影响。
将小鼠胚胎培养于对照培养基中，或实验1：加入FasL或FasL + SCF；实验2：加入过氧化氢（HP）或HP + SCF；实验3：进行冻融处理后，在有无SCF的情况下培养。对囊胚进行Fas和c-kit受体的免疫组织化学检测。测定囊胚率、囊胚细胞总数（TB）和内细胞团细胞计数（ICM）。
免疫组织化学研究显示囊胚细胞中Fas和c-kit均有表达。实验1：与FasL组和FasL + SCF组相比，对照组中发现的囊胚明显更多。与FasL组相比，对照组和FasL + SCF组的TB和ICM计数显著更高。实验2：在所有三个评估参数中，三组之间均存在显著差异。对照组的囊胚率、TB和ICM计数最高，HP + SCF组次之，HP组最差。实验3：在TB和ICM计数方面未发现显著差异。对照组中形成囊胚的胚胎比两个冷冻保存组更多。两个冷冻保存组之间的囊胚率没有差异。
SCF可能改善暴露于不利环境的胚胎的培养。
