Taniguchi Fuminori, Harada Tasuku, Nara Makoto, Deura Imari, Mitsunari Masahiro, Terakawa Naoki
Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishimachi, Yonago 683-8504, Japan.
J Assist Reprod Genet. 2004 Jun;21(6):223-8. doi: 10.1023/b:jarg.0000040238.61586.86.
To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells).
We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase-polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos.
The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage.
KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system.
评估人卵巢颗粒细胞瘤来源的细胞系(KGN细胞)与小鼠胚胎共培养的效果。
我们在显微镜下观察了小鼠植入前胚胎在与KGN细胞共培养系统中或在外源干细胞因子(SCF)存在的情况下的生长情况。采用逆转录聚合酶链反应(RT-PCR)方法分析与小鼠胚胎共培养的KGN细胞中SCF的基因表达。
与KGN细胞共培养系统显著提高了胚胎发育至晚期囊胚和孵化期的比率。我们还发现与小鼠胚胎共培养增强了KGN细胞中SCF的基因表达。向培养基中添加人重组SCF显著增强了胚胎发育至晚期囊胚和孵化期的能力。
KGN细胞可能通过SCF/c-kit旁分泌系统促进植入前胚胎发育。