Li Hua-Yu, Wang Shao-Min, Liu Hong-Min, Li Juan, Han Dong, Bu Shan-Shan, Zhang Ming-Zhi
Department of Chemistry, Zhengzhou University, 100 Science Road, 450001 Zhengzhou, China.
Rapid Commun Mass Spectrom. 2008 Oct;22(20):3161-71. doi: 10.1002/rcm.3721.
As modified nucleosides reflect altered tRNA turnover which seems to be impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High-performance liquid chromatography/electrosprary ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOFMS) was used to identify nucleosides purified from urine in positive ionization mode. Potential nucleosides were assessed by their evident UV absorbance in HPLC and then further examined by mass spectrometric techniques. In this manner, 21 nucleosides were detected in the urine of a patient with lymphoid cancer including three modified nucleosides 5'-dehydro-2-deoxyinosine, N1,N2,N7-trimethylguanosine and N1-methyl-N2-ethylguanosine, which had never been reported previously.
由于修饰核苷反映了tRNA周转的改变，而这在癌症患者体内似乎受到损害，因此它们已被评估为潜在的肿瘤标志物。采用高效液相色谱/电喷雾电离四极杆飞行时间质谱（HPLC/ESI-Q-TOFMS）在正离子模式下鉴定从尿液中纯化的核苷。通过其在HPLC中明显的紫外吸收来评估潜在的核苷，然后通过质谱技术进一步检测。通过这种方式，在一名淋巴癌患者的尿液中检测到21种核苷，包括三种修饰核苷5'-脱氢-2-脱氧肌苷、N1,N2,N7-三甲基鸟苷和N1-甲基-N2-乙基鸟苷，这些核苷此前从未有过报道。
