Tang Yi-wei, Luo Rong, Zhou Hui, Liu Wei, Mao Meng
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jul;39(4):540-3.
To investigate the relationship between Wnt pathway and intrauterine hypoxia-ischemia brain damage.
The gestational rats (15-17days) were randomly divided into sham operation group and transient intrauterine ischemia group. For transient intrauterine ischemia group, the gestational rats' bilateral uterine arteries were clamped for 30 min, followed by reperfusion. For sham operation, animals were subjected to the same surgical procedures, except that bilateral uterine arteries were not clamped. Samples were collected at 24, 48, and 72 h after reperfusion. The whole brain tissues were dissected for Nissl stain and Caspase-3 immumohistochemical. The expressions of GSK-3 beta and beta-catenin protein in the two groups were analyzed with Western Blotting.
The Nissl body of the transient intrauterine ischemia group decreased and the masculine cell of Caspase-3 was significantly higher than sham operation group at each time point (P < 0.05). The sham operation group is 12.71 +/- 1.42 (24 h), 11.45 +/- 1.50 (48 h), 14.10 +/- 1.29 (72 h) and the transient intrauterine ischemia group is 26.29 +/- 1.64 (24 h), 32.38 +/- 1.05 (48 h), 39.20 +/- 1.88 (72 h). beta-catenin expression between the two groups was no statistically significant difference. However, expression of GSK-3 beta in transient intrauterine ischemia group increased (P < 0.05). The sham operation group is 1.75 +/- 1.07 (24 h), 1.34 +/- 1.03 (48 h), 1.48 +/- 1.10 (72 h) and the transient intrauterine ischemia group is 3.25 +/- 1.34 (24 h), 2.99 +/- 1.26 (48 h), 3.10 +/- 1.35 (72 h).
Wnt pathway may play an important part in intrarterine hypoxia-ischemia brain damage.
探讨Wnt信号通路与宫内缺氧缺血性脑损伤之间的关系。
将孕龄15 - 17天的孕鼠随机分为假手术组和短暂性宫内缺血组。短暂性宫内缺血组，夹闭孕鼠双侧子宫动脉30分钟，然后再灌注。假手术组除不夹闭双侧子宫动脉外，接受相同的手术操作。再灌注后24、48和72小时采集样本。解剖全脑组织进行尼氏染色和Caspase-3免疫组织化学检测。采用蛋白质免疫印迹法分析两组中GSK-3β和β-连环蛋白的表达。
短暂性宫内缺血组尼氏体减少，各时间点Caspase-3阳性细胞均显著高于假手术组（P < 0.05）。假手术组分别为12.71±1.42（24小时）、11.45±1.50（48小时）、14.10±1.29（72小时），短暂性宫内缺血组分别为26.29±1.64（24小时）、32.38±1.05（48小时）、39.20±1.88（72小时）。两组间β-连环蛋白表达无统计学差异。然而，短暂性宫内缺血组GSK-3β表达增加（P < 0.05）。假手术组分别为1.75±1.07（24小时）、1.34±1.03（48小时）、1.48±1.10（72小时），短暂性宫内缺血组分别为3.25±1.34（24小时）、2.99±1.26（48小时）、3.10±1.35（72小时）。
Wnt信号通路可能在宫内缺氧缺血性脑损伤中起重要作用。
