Wang Gui-hua, Tang Xiao-hong, Ma Ya-li, Gao Yu-chun
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jul;39(4):575-8.
To investigate the effects of all-trans retinoic acid (ATRA) on the expression of transforming growth factor-beta 1 (TGF-beta 1) and collagen I (COL-I )in rat model of peritoneal dialysis, which may relate to the prevention peritoneal fibrosis.
Peritoneal dialysis model was established in rats, and then the rats were given ATRA 2 mg/kg (small dose group) or 5 mg/kg (large dose group) by the way of intraperitoneal injection once a day. All the rats were sacrificed on day 28. TGF-beta 1 and COL-I protein expression of peritoneum were measured by immunohistochemistry. TGF-beta 1 mRNA expression were examined with real time polymerase chain reaction (RT-PCR).
Masson stain showed that the peritoneum thickness was significantly increased in the rats model, and collagen deposition was evident in the thickened submesothelial compact zone. With the treatment of ATRA, either in small or large dose, pathological changes were significantly lessened. The expression of TGF-beta 1 and COL-I of peritoneum was increased significantly in the rats model, but the levels in the two ATRA treated groups were lower than those of the untreated group.
ATRA could decrease the experession of TGF-beta 1 and COL-I in peritoneum and delay the progression of peritoneal fibrosis.
探讨全反式维甲酸（ATRA）对大鼠腹膜透析模型中转化生长因子-β1（TGF-β1）和I型胶原（COL-I）表达的影响，这可能与预防腹膜纤维化有关。
建立大鼠腹膜透析模型，然后通过腹腔注射的方式，给予大鼠2mg/kg（小剂量组）或5mg/kg（大剂量组）的ATRA，每天一次。所有大鼠在第28天处死。采用免疫组织化学法检测腹膜中TGF-β1和COL-I蛋白表达。用实时聚合酶链反应（RT-PCR）检测TGF-β1 mRNA表达。
Masson染色显示，大鼠模型中腹膜厚度显著增加，增厚的间皮下致密区有明显的胶原沉积。经ATRA治疗，无论小剂量还是大剂量，病理变化均明显减轻。大鼠模型中腹膜TGF-β1和COL-I的表达显著增加，但两个ATRA治疗组的水平低于未治疗组。
ATRA可降低腹膜中TGF-β1和COL-I的表达，延缓腹膜纤维化的进展。
