Feng Renqing, Guo Zhenquan, Li Jinfeng
Department of Biochemistry, College of Life Sciences, Peking University, Beijing, P R China.
Prep Biochem Biotechnol. 2008;38(4):321-33. doi: 10.1080/10826060802325204.
Anti-GP-1D8 monoclonal antibody was produced in our laboratory. Immunoaffinity purification of GP-1D8 glycoprotein from human breast carcinoma tissues, on column with the monospecific antibody, is developed. The procedure permits purification of GP-1D8 to a highly purified state. It appeared as a single band in sodium dodcyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of GP-1D8 was determined to be 66 kDa by mass spectrometry. Its antigenicity was stable between 0 and 70 degrees C. It was breast cancer specific, as determined by immunohistochemistry and immunocytochemistry. The preparation of anti-GP-1D8 monoclonal antibody will facilitate further detection of, and functional study on, GP-1D8. The study also provides a simple, economical, and efficient method for affinity purification of target proteins from human or animal tissues.
抗GP-1D8单克隆抗体由我们实验室制备。我们开发了一种方法，使用该单特异性抗体柱从人乳腺癌组织中免疫亲和纯化GP-1D8糖蛋白。该方法可将GP-1D8纯化至高度纯化状态。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中它呈现为一条单一的条带。通过质谱法测定，GP-1D8的分子量为66 kDa。其抗原性在0至70摄氏度之间稳定。通过免疫组织化学和免疫细胞化学测定，它具有乳腺癌特异性。抗GP-1D8单克隆抗体的制备将有助于对GP-1D8进行进一步检测和功能研究。该研究还提供了一种从人或动物组织中亲和纯化目标蛋白的简单、经济且高效的方法。
