Wang Yong, Zhou Zong-guang, Xia Qing-jie, Zhang Wen-yan, Li Hong-guang, Wang Rong
Department of Digestive Surgery,West China Hospital, Sichuan University, Chengdu, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2008 Sep;11(5):465-8.
To investigate the expression differences of minichromosome maintenance 2 (MCM2) mRNA and protein among colon adenocarcinoma, colon adenoma and normal mucosa, and among different clinicopathological types of adenomas.
Fifty specimens, including 33 colonic adenomas, 12 colonic adenocarcinomas and 5 normal colonic mucosa were selected. Each specimen was divided into two parts, one for immunohistochemistry and the other for real-time RT-PCR. Expression differences of MCM2 mRNA among the colonic adenocarcinoma, adenoma and normal colonic mucosa were evaluated by REST-XL software.
The expression of MCM2 was observed in the basal third to half of the colonic crypts in normal mucosa, while throughout the epithelium in the colonic adenocarcinomas and adenomas. However, the expression of MCM2 mRNA in the adenocarcinomas was significantly higher than that in the adenomas(P=0.001). The MCM2 mRNA expression was elevated in the adenoma with villous type, in the conditions of high-grade dysplasia, larger size, sessile morphology and in patients of older ages, but the difference was not significant by REST-XL (P>0.05).
The difference of MCM2 expression between the adenoma and the adenocarcinoma indicates its potential value in the early diagnosis of colonic cancer.
探讨微小染色体维持蛋白2（MCM2）mRNA及蛋白在结肠腺癌、结肠腺瘤和正常黏膜组织中的表达差异，以及在不同临床病理类型腺瘤中的表达差异。
选取50例标本，其中包括33例结肠腺瘤、12例结肠腺癌及5例正常结肠黏膜。每个标本分为两部分，一部分用于免疫组织化学检测，另一部分用于实时逆转录聚合酶链反应（RT-PCR）。采用REST-XL软件评估结肠腺癌、腺瘤及正常结肠黏膜中MCM2 mRNA的表达差异。
正常黏膜组织中MCM2表达于结肠隐窝底部三分之一至二分之一处，而在结肠腺癌和腺瘤中MCM2表达于全层上皮。然而，结肠腺癌中MCM2 mRNA的表达显著高于结肠腺瘤（P = 0.001）。绒毛状类型腺瘤、高级别上皮内瘤变、肿瘤较大、无蒂形态及年龄较大患者的腺瘤中MCM2 mRNA表达升高，但REST-XL分析差异无统计学意义（P > 0.05）。
腺瘤与腺癌中MCM2表达的差异提示其在结肠癌早期诊断中的潜在价值。
