运用新型合成寡核苷酸探针原位杂交技术检测软骨肉瘤细胞中的FGF-β mRNA
Detection of FGF-beta mRNA in chondrosarcoma cells by a new in situ hybridization technique with synthetic oligonucleotide probes.
作者信息
Guthridge M, Bertolini J, Schmitt J, Hearn M T
机构信息
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
出版信息
J Biochem Biophys Methods. 1991 May-Jun;22(4):279-88. doi: 10.1016/0165-022x(91)90034-t.
PMID:1880334
Abstract
Fibroblast growth factor beta (FGF-beta) is a potent mitogenic and angiogenic factor produced by a large number of normal and transformed cells. In this paper we report a new application of the in situ hybridization procedure which has allowed the detection of FGF-beta mRNA in chondrosarcoma cells using 35S-labelled synthetic oligonucleotide probes.
摘要
成纤维细胞生长因子β（FGF-β）是一种由大量正常细胞和转化细胞产生的强效促有丝分裂和血管生成因子。在本文中，我们报告了原位杂交技术的一种新应用，该技术使用35S标记的合成寡核苷酸探针在软骨肉瘤细胞中检测到了FGF-β mRNA。
Zotero 插件