Eaf2的动态细胞内分布及其在紫外线诱导的DNA损伤反应中的潜在作用。
Dynamic intracellular distribution of Eaf2 and its potential involvement in UV-Induced DNA damage response.
作者信息
Zhuang Fengfeng, Yen Philip, Zhao Jiangyue, Nguyen Manuel, Jiang Meisheng, Liu Yi-Hsin
机构信息
Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA.
出版信息
DNA Cell Biol. 2008 Dec;27(12):649-56. doi: 10.1089/dna.2008.0733.
Abstract
Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. GFP-EAF2 is preferentially localized to the nucleus, and in the presence of ELL, it accumulates in nuclear speckles. However, Eaf2 is an unstable nuclear protein whose stability is affected by serum. Further, we provided first evidence that nuclear distribution of Eaf2 is responsive to DNA damage. Following UV irradiation, Eaf2 is relocalized to the nucleolus, suggesting a possible functional involvement of Eaf2 in DNA damage response.
摘要
Eaf2编码一种肿瘤抑制因子，该因子在转录激活、细胞凋亡和胚胎发育中发挥多种功能。在本研究中，我们利用绿色荧光蛋白（GFP）-Eaf2融合蛋白来描述Eaf2在亚细胞水平的动态移动。GFP-Eaf2优先定位于细胞核，并且在ELL存在的情况下，它会在核斑点中积累。然而，Eaf2是一种不稳定的核蛋白，其稳定性受血清影响。此外，我们首次证明Eaf2的核分布对DNA损伤有反应。紫外线照射后，Eaf2重新定位于核仁，这表明Eaf2可能参与DNA损伤反应。
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