Jin Hui, Wan Yun, Zhou Rui, Li Liangjun, Luo Rui, Zhang Sihua, Hu Junyong, Langford Paul R, Chen Huanchun
Division of Animal Infectious Disease in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Environ Microbiol. 2008 Dec;10(12):3326-36. doi: 10.1111/j.1462-2920.2008.01729.x. Epub 2008 Sep 16.
Haemophilus parasuis is the aetiological agent of Glässer's disease, which has received more attention in the past decade due to the increasing economic losses in the pig industry worldwide. Little is known about the mechanisms by which H. parasuis survives in the host. In this study, selective capture of transcribed sequences (SCOTS) was used to identify H. parasuis genes upregulated in necrotic porcine lung 7 days post infection. Thirty-eight genes were identified that were upregulated during infection of the lung tissue of pigs, compared with growth in culture medium. In two examples chosen gene expression was not confined to the lungs, there being variation between tissues. The data support biofilm formation being an important mode of growth for colonization and/or persistence. Results from the in vitro studies suggest that, as for other pathogens, iron and oxygen restriction and heat stress are important environmental signals to regulate gene expression. This study has identified genes of H. parasuis that are upregulated during infection of porcine lung tissue as compared with in vitro growth conditions.
副猪嗜血杆菌是格拉泽氏病的病原体,在过去十年中,由于全球养猪业经济损失不断增加,它受到了更多关注。关于副猪嗜血杆菌在宿主体内生存的机制,人们了解甚少。在本研究中,转录序列选择性捕获技术(SCOTS)被用于鉴定感染后7天在坏死猪肺中上调的副猪嗜血杆菌基因。与在培养基中生长相比,共鉴定出38个在猪肺组织感染期间上调的基因。在所选的两个例子中,基因表达并不局限于肺部,不同组织之间存在差异。数据支持生物膜形成是定植和/或持续存在的重要生长模式。体外研究结果表明,与其他病原体一样,铁和氧限制以及热应激是调节基因表达的重要环境信号。本研究已鉴定出与体外生长条件相比,在猪肺组织感染期间上调的副猪嗜血杆菌基因。