Kundrát Martin, Joss Jean M P, Smith Moya M
Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Evol Dev. 2008 Sep-Oct;10(5):531-6. doi: 10.1111/j.1525-142X.2008.00268.x.
Experimental evidence that the neural crest participates in tooth development in any osteichthyan fish has so far been lacking. Using vital dye cell-lineage tracking, we demonstrate that trigeminal stream neural crest cells contribute to the dental papilla of developing teeth in the Australian lungfish. Trigeminal neural crest cells labeled before migration have been traced during the earliest stages of tooth development. Neural crest cells from a single midbrain locus were relocated as ectomesenchyme in all developing teeth of the lungfish regardless of their topographical position in the dentition. These cells remain at the dental papilla interface and become cells committed to dentine production. Our findings provide the first cell-lineage evidence that cranial neural crest is fated to ectomesenchyme for tooth development and dentine production in the living sister-group to tetrapods. This shows that cranial neural crest contribution to teeth is conserved from this node on the tetrapod phylogeny.
迄今为止，尚未有实验证据表明硬骨鱼类的神经嵴参与牙齿发育。我们通过活体染料细胞谱系追踪证明，三叉神经流神经嵴细胞对澳大利亚肺鱼发育中牙齿的牙乳头有贡献。迁移前标记的三叉神经嵴细胞在牙齿发育的最早阶段就已被追踪。来自单个中脑位点的神经嵴细胞作为外胚间充质重新定位到肺鱼所有发育中的牙齿中，无论它们在牙列中的拓扑位置如何。这些细胞保留在牙乳头界面，并成为致力于牙本质生成的细胞。我们的研究结果提供了首个细胞谱系证据，表明在四足动物现存的姐妹类群中，颅神经嵴注定要分化为外胚间充质以进行牙齿发育和牙本质生成。这表明从四足动物系统发育的这个节点开始，颅神经嵴对牙齿的贡献是保守的。
