将两个酶基因座定位到四斑按蚊A种的X染色体上。

Assignment of two enzyme loci to the X chromosome of Anopheles quadrimaculatus species A.

作者信息

Lanzaro G C, Mitchell S E, Narang S K, Seawright J A

机构信息

Department of Entomolgy and Nematology, University of Florida, Gainesville.

出版信息

J Hered. 1991 Jul-Aug;82(4):349-51. doi: 10.1093/oxfordjournals.jhered.a111098.

DOI:10.1093/oxfordjournals.jhered.a111098

PMID:1880395

Abstract

Analysis of isozyme variability in four natural populations of Anopheles quadrimaculatus Species A indicated that the loci for Malic enzyme (Me) and Mannose phosphate isomerase-1 (Mpi-1) are on the X chromosome. There were female heterozygotes, but no male heterozygotes were observed. Strains fixed for fast- and slow-migrating allozymes were devised and crossed. Progeny phenotypes conformed to expectations for sex linkage; female progeny were heterozygous, and male progeny were hemizygous for the maternal allele. The three-point cross, using the Me and Mpi-1 loci with the sex-linked mutant rose eye (ro), established the gene sequence Mpi-1-11.1-Me-40.8-ro.

摘要

对四斑按蚊A种四个自然种群的同工酶变异性分析表明，苹果酸酶（Me）和磷酸甘露糖异构酶-1（Mpi-1）的基因座位于X染色体上。存在雌性杂合子，但未观察到雄性杂合子。设计并杂交了固定有快速和慢速迁移同工酶的品系。后代的表型符合性连锁的预期；雌性后代是杂合子，雄性后代对于母本等位基因是半合子。利用Me和Mpi-1基因座与性连锁突变体玫瑰眼（ro）进行三点杂交，确定了基因序列Mpi-1-11.1-Me-40.8-ro。