Thanaphum S, Green C A, Baimai V, Gass R F, Gingrich J B
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Genetica. 1990;82(1):63-72. doi: 10.1007/BF00057676.
Two laboratory stocks of Anopheles minimus, each fixed for variant electromorphs of esterases, aspartate aminotransferase, hydroxyacid dehydrogenase, phosphogluconate dehydrogenase, mannose phosphate isomerase and glycerol dehydrogenase were used to assess linkage relationships between presumed gene loci controlling this variation. The two F1, which had been obtained from crossing the stocks, were backcrossed to a parental stock. Three loci controlled the esterases and one locus each of the other enzymes. Mpi is sex-linked. The rest are autosomal and suggested relationships are: Pgd 2.3% recombination from Aat and unlinked to any other loci; Est-1-33.8%-Est-3-31.5%-Est-2-21.0%-Had. Gcd is unlinked to any other locus. There was evidence of strong interaction between the X chromosome of one stock and autosomes of the other in which individuals bearing the X chromosome of the one suffered relatively greater mortality and had delayed development with respect to other genotypic classes.
使用两种微小按蚊实验室种群，每种种群在酯酶、天冬氨酸转氨酶、羟酸脱氢酶、磷酸葡萄糖酸脱氢酶、甘露糖磷酸异构酶和甘油脱氢酶的变异电泳形态上是固定的，以评估控制这种变异的假定基因座之间的连锁关系。从两种种群杂交获得的两个F1代与一个亲本种群进行回交。三个基因座控制酯酶，其他每种酶各受一个基因座控制。Mpi是性连锁的。其余的是常染色体的，推测的关系是：Pgd与Aat的重组率为2.3%，且与任何其他基因座不连锁；Est-1-33.8%-Est-3-31.5%-Est-2-21.0%-Had。Gcd与任何其他基因座不连锁。有证据表明，一种种群的X染色体与另一种种群的常染色体之间存在强烈的相互作用，其中携带一种种群X染色体的个体相对于其他基因型类别死亡率相对较高且发育延迟。
