Larsen Tine Lise, Nilsen Valentina, Andersen Dag Olav, Francis George, Rustad Pål, Mansoor Mohammad Azam
Department of Natural Sciences, University of Agder, 25 J Gimlemoen, Service box 422, 4604 Kristiansand, Norway.
Clin Biochem. 2008 Dec;41(18):1474-81. doi: 10.1016/j.clinbiochem.2008.08.083. Epub 2008 Sep 9.
Bioavailability of NO can be estimated by measuring the concentration of nitrate (NO(3)) in serum. However, the methods used for the measurement NO(3) in plasma or serum show a great degree of variation. Therefore, we compared two analytical methods for the measurement of NO(3) in serum.
The concentration of NO(3) in 600 serum samples collected from healthy individuals was determined by the HPLC and by the Griess reagent-spectroscopic method.
The concentration of NO(3) in the samples was 29.4+/-16.1 micromol/L and 26.2+/-14.0 micromol/L (mean+/-SD) measured by HPLC and Griess reagent-spectroscopic method respectively (p<0.0001). We detected a significant correlation between the two methods (R=0.81, p<0.0001).
A significant correlation between the two methods may suggest that either method can be used for the measurement of NO(3) in serum, however the Griess reagent-spectroscopic method measures lower concentrations of NO(3) than the HPLC method.
一氧化氮（NO）的生物利用度可通过测量血清中硝酸盐（NO₃）的浓度来估算。然而，用于测量血浆或血清中NO₃的方法存在很大差异。因此，我们比较了两种分析方法来测量血清中的NO₃。
采用高效液相色谱法（HPLC）和格里斯试剂光谱法测定了从健康个体采集的600份血清样本中NO₃的浓度。
通过HPLC法和格里斯试剂光谱法测得样本中NO₃的浓度分别为29.4±16.1微摩尔/升和26.2±14.0微摩尔/升（平均值±标准差）（p<0.0001）。我们检测到两种方法之间存在显著相关性（R = 0.81，p<0.0001）。
两种方法之间的显著相关性可能表明两种方法均可用于测量血清中的NO₃，然而格里斯试剂光谱法测得的NO₃浓度低于HPLC法。
