Li Lei, Baibakov Boris, Dean Jurrien
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
Dev Cell. 2008 Sep;15(3):416-425. doi: 10.1016/j.devcel.2008.07.010.
We have identified a subcortical maternal complex (SCMC) that assembles during oocyte growth and is essential for zygotes to progress beyond the first embryonic cell divisions. At least four maternally encoded proteins contribute to this MDa complex: FLOPED, MATER, and TLE6 interact with each other while Filia binds independently to MATER. Although the transcripts encoding these proteins are degraded during meiotic maturation and ovulation, the SCMC proteins persist in the early embryo. The SCMC, located in the subcortex of eggs, is excluded from regions of cell-cell contact in the cleavage-stage embryo and segregates to the outer cells of the morulae and blastocyst. Floped(tm/tm) and/or Mater(tm/tm) eggs lack the SCMC but can be fertilized. However, these embryos do not progress beyond cleavage stage development and female mice are sterile. The proteins are conserved in humans, and similar maternal effect mutations may result in recurrent embryonic loss.
我们已经鉴定出一种皮质下母体复合体（SCMC），它在卵母细胞生长过程中组装，对于合子在第一次胚胎细胞分裂后继续发育至关重要。至少有四种母体编码的蛋白质参与了这个MDa复合体：FLOPED、MATER和TLE6相互作用，而Filia则独立与MATER结合。尽管编码这些蛋白质的转录本在减数分裂成熟和排卵过程中会降解，但SCMC蛋白质在早期胚胎中持续存在。SCMC位于卵子的皮质下，在卵裂期胚胎的细胞间接触区域被排除，并分离到桑葚胚和囊胚的外层细胞中。Floped(tm/tm)和/或Mater(tm/tm)卵子缺乏SCMC，但可以受精。然而，这些胚胎在卵裂期发育后无法继续发育，雌性小鼠不育。这些蛋白质在人类中是保守的，类似的母体效应突变可能导致反复的胚胎丢失。
