通过鲁米诺/过氧化物酶快速停流化学发光抑制法测定杀草强。

Determination of asulam by fast stopped-flow chemiluminescence inhibition of luminol/peroxidase.

作者信息

García Sánchez F, Navas Díaz A, Delgado Téllez C, Algarra M

机构信息

Department of Analytical Chemistry, Faculty of Sciences, University of Málaga, Campus de Teatinos s/n, 29071 Málaga, Spain. f_

出版信息

Talanta. 2008 Oct 19;77(1):294-7. doi: 10.1016/j.talanta.2008.06.025. Epub 2008 Jun 27.

DOI:10.1016/j.talanta.2008.06.025

PMID:18804636

Abstract

An efficient, sensitive and fast stopped-flow method has been developed to determine asulam in water, based on its inhibition effect on the horseradish peroxidase-luminol-hydrogen peroxide chemiluminescence reaction, (HRP-luminol-H(2)O(2)). Ultra fast data acquisition (0.20s) facilitates excellent selectivity because no interferences from concomitants in the matrix act in such short time scale. The precision as repeatability (expressed as relative standard deviation, n=10) was 0.4% at a 40 pM level. The detection limit was 1.5 pM (0.35 ng/L) and 7.15 pM in pure and raw water, respectively. The calibration data over the range 5-60 pM present a correlation coefficient of r=0.9993. The proposed method has been applied to determine asulam in water samples by using solid-phase extraction (SPE). Mean recovery value was 98.1+/-2% at 50 pM level.

摘要

基于水中杀草强对辣根过氧化物酶-鲁米诺-过氧化氢化学发光反应（HRP-luminol-H₂O₂）的抑制作用，开发了一种高效、灵敏且快速的停流法来测定水中的杀草强。超快速数据采集（0.20秒）有助于实现出色的选择性，因为在如此短的时间尺度内，基质中的共存物不会产生干扰。在40 pM水平下，重复性精密度（以相对标准偏差表示，n = 10）为0.4%。在纯水和原水中，检测限分别为1.5 pM（0.35 ng/L）和7.15 pM。5 - 60 pM范围内的校准数据的相关系数r = 0.9993。所提出的方法已应用于通过固相萃取（SPE）测定水样中的杀草强。在50 pM水平下，平均回收率为98.1±2%。