Jesus Desyreé Murta, Moussatché Nissin, Damaso Clarissa R
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - CCS, Ilha do Fundão, Rio de Janeiro 21941-590, Brazil.
Int J Antimicrob Agents. 2009 Jan;33(1):75-9. doi: 10.1016/j.ijantimicag.2008.07.015. Epub 2008 Sep 19.
The antiviral effect of cidofovir was evaluated against two strains of vaccinia virus: the field strain Cantagalo virus (CTGV) and the smallpox vaccine IOC. The drug severely inhibited virus replication, revealing an EC(50) (drug concentration required to inhibit 50% of virus replication) of 7.68 microM and 9.66 microM, respectively, for CTGV and vaccine strain IOC. Similarly, other field isolates of Cantagalo-like viruses recently collected in distinct outbreaks were equally sensitive to the drug. Pre-treatment of cells prior to infection effectively established an antiviral state, inhibiting virus replication by >90% after 24h in the absence of cidofovir. CTGV infections represent an emerging zoonosis, and outbreaks have been frequently reported in several states of Brazil. Also, the possibility of resuming the manufacture of smallpox vaccine supports the need to evaluate the effect of antiviral drugs on the Brazilian vaccine strain IOC. As there is no currently approved antipoxvirus therapy, our data are extremely encouraging.
野外毒株坎塔加洛病毒（CTGV）和天花疫苗IOC。该药物严重抑制病毒复制，CTGV和疫苗株IOC的半数有效浓度（抑制50%病毒复制所需的药物浓度）分别为7.68微摩尔和9.66微摩尔。同样，最近在不同疫情中收集的其他类似坎塔加洛病毒的野外分离株对该药物同样敏感。在感染前对细胞进行预处理有效地建立了抗病毒状态，在没有西多福韦的情况下，24小时后病毒复制被抑制超过90%。CTGV感染是一种新出现的人畜共患病，在巴西的几个州经常有疫情报告。此外，恢复天花疫苗生产的可能性支持了评估抗病毒药物对巴西疫苗株IOC效果的必要性。由于目前没有批准的抗痘病毒疗法，我们的数据非常令人鼓舞。
