Central Institute for Research on Goats, Makhdoom, Mathura, UP, India.
Comp Immunol Microbiol Infect Dis. 2010 Mar;33(2):145-59. doi: 10.1016/j.cimid.2008.08.006. Epub 2008 Sep 20.
Information on Mycobacterium avium subspecies paratuberculosis (MAP) genotypes infecting different animal species in India is limited. Presence of MAP was investigated in free ranging antelopes (locally known as Nilgai/blue bulls/Boselaphus tragocamelus) using direct microscopy, culture, IS900 PCR and IS1311 PCR-REA. IS900 elements of MAP from Nilgai and previously isolated from goats were sequenced and compared to establish inter-species transmission between free ranging Nilgai and closed farm herds and flocks of goats and sheep sharing common grazing and water resources. Fecal samples were collected from two geographical regions (Mathura and Kanpur Dehat districts) separated by 300km, in North India. Of the 42 fecal samples cultured, MAP colonies were recovered from 23.8% samples (Nilgai). Of the 10 positive fecal samples, two were in 'Super shedder' (>1000cfu/g) category and rest were moderate (<10-100cfu/g) shedders. None of the Nilgai from Kanpur Dehat was positive in culture. The 229bp fragment targeting specific IS900 sequence was amplified from template DNA isolated from all the positive MAP cultures of Nilgai. Using IS1311 PCR-REA, MAP colonies were genotyped as 'Bison type'. Goatherds and a sheep flock located at Central Institute for Research on Goats (CIRG), shared 303.52ha of land (Mathura district of Uttar Pradesh) with Nilgai and were endemic for MAP infection. MAP strains isolated from goats and sheep have been genotyped as 'Bison type'. Nucleotide sequence of the insertion elements (900) from MAP 'Bison type' strain (S5) of goat origin and MAP (B42) from Nilgai showed difference of 2 (1%) base pairs at the 11th and 12th position (Genbank accession number EU130943). Study is first report on sharing (inter-species transmission) of a new 'Bison type' genotype of MAP between free ranging wildlife (Nilgai population) and domestic animals (farm goatherds and sheep flocks) in India.
关于感染印度不同动物物种的鸟分枝杆菌亚种副结核分枝杆菌（MAP）基因型的信息有限。本研究使用直接显微镜检查、培养、IS900 PCR 和 IS1311 PCR-REA 方法调查了自由放养的羚羊（当地称为印度野牛/蓝牛/Boselaphus tragocamelus）中的 MAP 存在情况。对来自印度野牛和之前从山羊中分离出的 MAP 的 IS900 元件进行测序，并进行比较，以确定自由放养的印度野牛与封闭的农场牛群以及共享共同放牧和水资源的山羊和绵羊群之间的种间传播。从印度北部的两个地理区域（马图拉和坎普尔德哈特区）收集了 42 份粪便样本。在培养的 42 份粪便样本中，MAP 菌落从 23.8%的样本（印度野牛）中回收。在 10 个阳性粪便样本中，有 2 个属于“超级排放者”（>1000cfu/g）类别，其余为中度（<10-100cfu/g）排放者。来自坎普尔德哈特的印度野牛中没有一个在培养中呈阳性。从所有印度野牛的阳性 MAP 培养物中分离出的模板 DNA 中扩增出了针对特定 IS900 序列的 229bp 片段。使用 IS1311 PCR-REA，MAP 菌落被基因分型为“野牛型”。位于中央山羊研究所（CIRG）的山羊放牧者和一个绵羊群与印度野牛共享 303.52 公顷土地（北方邦马图拉区），并存在 MAP 感染。从山羊和绵羊中分离出的 MAP 菌株已被基因分型为“野牛型”。来自山羊源 MAP“野牛型”菌株（S5）和印度野牛 MAP（B42）的插入元件（900）的核苷酸序列在第 11 和第 12 位有 2（1%）个碱基的差异（Genbank 登录号 EU130943）。该研究首次报道了在印度，自由放养的野生动物（印度野牛种群）和家畜（农场山羊和绵羊群）之间共享（种间传播）一种新的“野牛型”MAP 基因型。
