Microbiology Laboratory, Central Institute for Research on Goats, Makhdoom, PO-FARAH, District Mathura (UP), India.
Microbiol Res. 2010 Feb 28;165(2):163-71. doi: 10.1016/j.micres.2009.03.007. Epub 2009 May 12.
Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of the etiological agent at phenotypic and molecular levels. On the basis of cultural phenotypes and IS1311 PCR-REA typing, MAP strains have been genotyped as 'Cattle type', 'Sheep type' and 'Bison type'. Information exists on genetic differences and comparative evolution of 'Cattle type' and 'Sheep type' strains after divergence from M. avium; however, emphasis has been little on 'Bison type' strains. Recently, a new 'Indian Bison type' genotype has been reported as principal strain infecting different animal species and human beings in India. The study analyzed few genetic markers to have inferences on the molecular evolution of native MAP isolates belonging to 'Bison type' genotype. Results pointed towards recent evolution of 'Bison type' genotype.
分支杆菌亚种副结核分枝杆菌（MAP）是动物中约翰氏病（JD）的病原体，也与人类的克罗恩病有关。缺乏本土诊断和疫苗阻碍了印度 JD 的控制。设计有效的控制策略需要在表型和分子水平上彻底了解病因。根据文化表型和 IS1311 PCR-REA 分型，MAP 菌株被分为“牛型”、“羊型”和“野牛型”。在与分枝杆菌分枝杆菌（M. avium）分化后，已经存在关于“牛型”和“羊型”菌株遗传差异和比较进化的信息；然而，对“野牛型”菌株的关注较少。最近，据报道，一种新的“印度野牛型”基因型是印度主要感染不同动物物种和人类的菌株。该研究分析了一些遗传标记，以推断属于“野牛型”基因型的本土 MAP 分离株的分子进化。结果表明“野牛型”基因型是最近进化而来的。
