SigF控制耻垢分枝杆菌中类胡萝卜素色素的产生，并影响其转化效率和对过氧化氢的敏感性。
SigF controls carotenoid pigment production and affects transformation efficiency and hydrogen peroxide sensitivity in Mycobacterium smegmatis.
作者信息
Provvedi Roberta, Kocíncová Dana, Donà Valentina, Euphrasie Daniel, Daffé Mamadou, Etienne Gilles, Manganelli Riccardo, Reyrat Jean-Marc
机构信息
Dipartimento di Biologia, Universitá di Padova, Padua, Italy.
出版信息
J Bacteriol. 2008 Dec;190(23):7859-63. doi: 10.1128/JB.00714-08. Epub 2008 Sep 19.
Abstract
Carotenoids are complex lipids that are known for acting against photodynamic injury and free radicals. We demonstrate here that sigma(F) is required for carotenoid pigment production in Mycobacterium smegmatis. We further show that a sigF mutant exhibits a transformation efficiency 10(4)-fold higher than that of the parental strain, suggesting that sigma(F) regulates the production of components affecting cell wall permeability. In addition, a sigF mutant showed an increased sensitivity to hydrogen peroxide. An in silico search of the M. smegmatis genome identified a number of SigF consensus sites, including sites upstream of the carotenoid synthesis locus, which explains its SigF regulation.
摘要
类胡萝卜素是一类复杂的脂质，以其抗光动力损伤和自由基的作用而闻名。我们在此证明，耻垢分枝杆菌中类胡萝卜素色素的产生需要σ(F)。我们进一步表明，sigF突变体的转化效率比亲本菌株高10^4倍，这表明σ(F)调节影响细胞壁通透性的成分的产生。此外，sigF突变体对过氧化氢的敏感性增加。对耻垢分枝杆菌基因组进行的电子搜索确定了许多SigF共有位点，包括类胡萝卜素合成基因座上游的位点，这解释了其SigF调控机制。
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