Paul Angela, Straub Andreas, Weber Norbert, Ziemer Gerhard, Wendel Hans Peter
Department of Thoracic, University Hospital Tuebingen, Calwerstr. 7/1, 72076, Tuebingen, Germany.
J Mater Sci Mater Med. 2009 Jan;20(1):373-8. doi: 10.1007/s10856-008-3587-y. Epub 2008 Sep 21.
Cardiopulmonary bypass (CPB) surgery is associated with platelet activation and reduced platelet counts due to artificial surface activation of blood elements and non-physiological flow-patterns. As shown in former studies, coating of medical devices can improve hemocompatibility in extracorporeal circulation systems. In this study, we demonstrate a new method to determine platelet adhesion on 18 coated and non-coated membrane oxygenators in a simulated CPB model with CD41 Western blot. Platelet loss and the release of beta-TG (platelet activation marker) were determined during a 120 min recirculation phase. At the end of the run the membrane oxygenators (with tubing system) were rinsed and the amount of adsorbed proteins on the surface was analyzed by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting technique. Uncoated devices showed significantly higher concentrations of CD41 and of fibrinogen adsorption compared to the coated membrane oxygenators. These results correspond with the release of beta-TG and platelet loss indicating less platelet adhesion on the coated oxygenators compared with the uncoated group. This new method may be useful in choosing less platelet activating materials for all kind of blood contacting devices to improve thrombogenicity including platelet functionality.
体外循环（CPB）手术会导致血小板活化以及血小板计数减少，这是由于血液成分的人工表面活化和非生理血流模式所致。如先前研究所示，医疗器械的涂层可改善体外循环系统中的血液相容性。在本研究中，我们展示了一种新方法，即在模拟CPB模型中，使用CD41免疫印迹法测定18种有涂层和无涂层的膜式氧合器上的血小板黏附情况。在120分钟的再循环阶段测定血小板损失和β-血小板球蛋白（血小板活化标志物）的释放情况。实验结束时，冲洗膜式氧合器（连同管路系统），并使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹技术分析表面吸附蛋白的量。与有涂层的膜式氧合器相比，无涂层装置显示出显著更高的CD41浓度和纤维蛋白原吸附量。这些结果与β-血小板球蛋白的释放和血小板损失情况相符，表明与未涂层组相比，有涂层的氧合器上的血小板黏附较少。这种新方法可能有助于为各类血液接触装置选择血小板活化作用较小的材料，以改善包括血小板功能在内的血栓形成性。
