Ghosh A S, Bhattacharyya D, Chandra M, Bhattacharyya T K
Department of Chemical Technology, University College of Science and Technology, Calcutta University, 92, A.P.C.Road, Kolkata.
Indian J Exp Biol. 2008 Jul;46(7):498-504.
Hepatocarcinogenesis was induced in Sprague Dawley rats by injecting diethylnitrosamine (DENA); 150 mg/kg body weight, ip, a well known liver carcinogen and a mutagenic agent. Concurrent with the induction of hepatocarcinoma, psychological stress was also elicited from the changes in brain neurotransmitters. Noradrenaline and dopamine, the neurotransmitters of sympathetic system were estimated from the whole brain and corresponding hormones T3, T4 and prolactin were estimated from the blood of such rats. The neuroendocrine cascade and the marker enzyme gamma glutamyl transferase were estimated at 7, 14, 21 and 30 weeks. A direct relationship between noradrenaline, T3 and T4 and a reciprocal relationship between dopamine and prolactin were observed, which may be correlated to the carcinogenic effect of DENA.
通过腹腔注射二乙基亚硝胺（DENA）（150毫克/千克体重，一种著名的肝脏致癌物和诱变剂）诱导斯普拉格-道利大鼠发生肝癌。在诱导肝癌的同时，脑内神经递质的变化也引发了心理应激。从全脑中测定交感神经系统的神经递质去甲肾上腺素和多巴胺，并从这些大鼠的血液中测定相应的激素三碘甲状腺原氨酸（T3）、甲状腺素（T4）和催乳素。在第7、14、21和30周时测定神经内分泌级联反应和标记酶γ-谷氨酰转移酶。观察到去甲肾上腺素、T3和T4之间存在直接关系，多巴胺和催乳素之间存在相互关系，这可能与DENA的致癌作用相关。
