Wang Song-Hua, Zhang Hua, Cui Yuan-Rong, He Qing-Yuan, Zhang Qiang
College of Life Science, Anhui Science and Technology University, Bengbu 233100, Anhui, China.
Ying Yong Sheng Tai Xue Bao. 2008 Jun;19(6):1355-61.
The study on the effects of different concentration (0, 10, 50, 100, 200 and 400 micromol x L(-1)) cadmium (Cd) on the antioxidative system in Ganoderma lucidum mycelia indicated that with increasing concentration of Cd, the fresh mass and the proline, total polysaccharides, and reduced polysaccharides contents of G. lucidum mycelia decreased, but non-protein thiol (NPT) content increased. At 400 micromol x L(-1) of Cd, the NPT content increased dramatically, being 5.7 times higher than control. Within the range of test Cd concentrations, the activities of CAT, GR and POD increased first and decreased then, with the peak at 100 micromol x L(-1) of Cd, while the activities of LOX and SOD increased with increasing Cd concentration, with the maximum at 400 micromol x L(-1) of Cd. Polyacrylamide gel electrophoresis analysis revealed that 100-400 micromol x L(-1) of Cd induced two additional isozymes bands of Mn-SOD, 10-200 micromol x L(-1) of Cd increased the intensity of constitutive isozymes of CAT, POD, SOD and LOX, while 400 micromol x L(-1) of Cd decreased the intensity of isozymes of POD significantly.
研究不同浓度（0、10、50、100、200和400微摩尔×升⁻¹）镉（Cd）对灵芝菌丝体抗氧化系统的影响，结果表明，随着Cd浓度的增加，灵芝菌丝体的鲜质量、脯氨酸、总多糖和还原多糖含量降低，但非蛋白硫醇（NPT）含量增加。在Cd浓度为400微摩尔×升⁻¹时，NPT含量急剧增加，比对照高5.7倍。在测试的Cd浓度范围内，CAT、GR和POD的活性先升高后降低，在Cd浓度为100微摩尔×升⁻¹时达到峰值，而LOX和SOD的活性随Cd浓度的增加而升高，在Cd浓度为400微摩尔×升⁻¹时达到最大值。聚丙烯酰胺凝胶电泳分析显示，100 - 400微摩尔×升⁻¹的Cd诱导出两条额外的Mn - SOD同工酶带，10 - 200微摩尔×升⁻¹的Cd增加了CAT、POD、SOD和LOX组成型同工酶的强度，而400微摩尔×升⁻¹的Cd显著降低了POD同工酶的强度。
