Schiller Alexander, Vilozny Boaz, Wessling Ritchie A, Singaram Bakthan
Empa - Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Protection and Physiology, Lerchenfeldstr. 5, 9014 St. Gallen, Switzerland.
Anal Chim Acta. 2008 Oct 10;627(2):203-11. doi: 10.1016/j.aca.2008.08.011. Epub 2008 Aug 23.
The solution-phase sensor array of three cationic bis-boronic acid appended benzyl viologens (BBV) and the anionic fluorescent dye, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS), is able to discriminate among five phospho sugars, four nucleotides and three neutral saccharides in aqueous buffered solution at low mM concentrations. Linear discriminant analysis, principal component analysis, and hierachical cluster analysis studies showed the "discrimination limit" (lowest analyte concentration where the discrimination is still 100%) to be 4mM. Calculated K(b) and F(max)/F(0) values from binding curves of the three BBVs with 1-12 were also used to perform multi-variate analyses with very good discrimination results.
由三种阳离子型双硼酸苄基紫精（BBV）和阴离子型荧光染料8-羟基芘-1,3,6-三磺酸三钠盐（HPTS）组成的溶液相传感器阵列，能够在低毫摩尔浓度的水性缓冲溶液中区分五种磷酸糖、四种核苷酸和三种中性糖。线性判别分析、主成分分析和层次聚类分析研究表明，“判别极限”（仍能实现100%判别的最低分析物浓度）为4 mM。根据三种BBV与1-12的结合曲线计算出的K(b)和F(max)/F(0)值，也用于进行多变量分析，判别结果非常好。
