Zeng Yan, Zhang Guanxin, Zhang Deqing
Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Anal Chim Acta. 2008 Oct 10;627(2):254-7. doi: 10.1016/j.aca.2008.08.028. Epub 2008 Aug 29.
Compound 1 as an electron donor-acceptor compound with N,N-dimethylaniline and quinone units was designed for a highly selective colorimetric determination of thiol-containing amino acids and peptides, by making use of the unique reactivity of thiol towards quinone. Compound 1 shows a strong intramolecular charge transfer (ICT) band around 582nm; but, it decreased after addition of either cysteine (Cys) or glutathione (GSH). Moreover, the ICT band intensity at 582nm decreased linearly with the increasing concentrations of Cys or GSH. The interference from other amino acids can be neglected. Therefore, compound 1 can be employed as a selective colorimetric visual chemosensor for thiol-containing amino acids and peptides.
化合物1作为一种具有N，N - 二甲基苯胺和醌单元的电子供体 - 受体化合物，利用硫醇对醌的独特反应性，被设计用于高选择性比色测定含硫醇的氨基酸和肽。化合物1在582nm左右显示出很强的分子内电荷转移（ICT）带；但是，加入半胱氨酸（Cys）或谷胱甘肽（GSH）后该带减弱。此外，582nm处的ICT带强度随Cys或GSH浓度的增加呈线性下降。其他氨基酸的干扰可以忽略不计。因此，化合物1可作为含硫醇的氨基酸和肽的选择性比色视觉化学传感器。
