Nakazawa Kohji, Izumi Yumiko, Mori Ryuhei
Department of Chemical Processes and Environments, University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
Acta Biomater. 2009 Feb;5(2):613-20. doi: 10.1016/j.actbio.2008.08.011. Epub 2008 Sep 5.
This study describes the morphological and functional behavior of rat hepatocytes on a polydimethylsiloxane (PDMS)-coated surface. Hepatocytes were cultured on hydrophobic or hydrophilic PDMS-coated surfaces in serum-free and serum-containing media. In the serum-free medium, almost all hepatocytes adhered onto the surface irrespective of the wettability, with a cell adhesion ratio of >90% at 24h. In the serum-containing medium, although they strongly adhered onto the hydrophilic surface (cell adhesion ratio >85%), the ratio on the hydrophobic surface was <15%. Furthermore, hepatocytes in the serum-free medium gradually formed spheroids irrespective of the surface characteristics; however, on the hydrophilic surface in the serum-containing medium, they maintained a monolayer configuration for up to 10 days, and their numbers gradually decreased over time. Expression levels of the functional activities (albumin secretion and ammonia removal) and the cell-cell adhesion molecules (cadherin and connexin-32) were higher in the hepatocytes that formed spheroids compared to those which assumed a monolayer configuration, and these levels were maintained for at least 10 days. These results suggest that the wettability of PDMS and the composition of the culture medium together control the cell adhesion, morphology and expression of functional genes in hepatocytes.
本研究描述了大鼠肝细胞在聚二甲基硅氧烷（PDMS）涂层表面的形态和功能行为。肝细胞在无血清和含血清培养基中，于疏水或亲水的PDMS涂层表面进行培养。在无血清培养基中，几乎所有肝细胞均能黏附于表面，与表面润湿性无关，在24小时时细胞黏附率>90%。在含血清培养基中，虽然它们能强烈黏附于亲水表面（细胞黏附率>85%），但在疏水表面的黏附率<15%。此外，无血清培养基中的肝细胞无论表面特性如何均逐渐形成球体；然而，在含血清培养基中的亲水表面上，它们在长达10天的时间内维持单层结构，且其数量随时间逐渐减少。与呈单层结构的肝细胞相比，形成球体的肝细胞中功能活性（白蛋白分泌和氨清除）及细胞间黏附分子（钙黏蛋白和连接蛋白-32）的表达水平更高，且这些水平至少维持10天。这些结果表明，PDMS的润湿性和培养基的成分共同控制着肝细胞的细胞黏附、形态及功能基因的表达。
