[大鼠小肠黏膜中谷胱甘肽S-转移酶同工酶的分布]

[Distribution of glutathione S-transferase isozymes in rat small intestinal mucosa].

作者信息

Yamamuro W, Saiki H, Sugimoto M

机构信息

Second Department of Internal Medicine, Toho University School of Medicine, Tokyo.

出版信息

Nihon Shokakibyo Gakkai Zasshi. 1991 May;88(5):1184-90.

PMID:1880950

Abstract

We have investigated the longitudinal distribution of glutathione S-transferase (GST) isozymes in the trisected small intestine mucosa of rats. Only GST subunits 1 and 7 were detected by Western blot analysis of the intestinal cytosol using antiserum for GST1-1, 1-2, 3-4 and 7-7. Cytosolic GST1-1, 3-4 and 7-7 were assayed by the quantitative ELISA. There was a marked decline of the concentration of GST1-1 from proximal (35.17 nmol/g tissue) to distal intestine (1.67 nmol/g tissue). GST3-4 was hardly detected in the intestinal mucosa. Among the GSTs, GST7-7 existed in the highest concentration in any segment of intestine, i.e. 58.76 nmol/g tissue (61% of GSTs) in the proximal intestine and 32.38 nmol/g tissue (93% of GSTs) in the distal intestine.

摘要

我们研究了大鼠三段式小肠黏膜中谷胱甘肽S-转移酶（GST）同工酶的纵向分布。使用针对GST1-1、1-2、3-4和7-7的抗血清对肠细胞溶质进行蛋白质免疫印迹分析，仅检测到GST亚基1和7。通过定量酶联免疫吸附测定法检测细胞溶质中的GST1-1、3-4和7-7。GST1-1的浓度从近端小肠（35.17 nmol/g组织）到远端小肠（1.67 nmol/g组织）显著下降。在肠黏膜中几乎检测不到GST3-4。在所有GST中，GST7-7在小肠的任何节段中浓度最高，即近端小肠中为58.76 nmol/g组织（占GST的61%），远端小肠中为32.38 nmol/g组织（占GST的93%）。