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Epigenetic deregulation of the human Oct4 promoter in mouse cells.

作者信息

Cha Young, Sung Min-Kyung, Jung Kyung-Won, Kim Hwan-Hee, Lee Su-Man, Park Kyung-Soon

机构信息

Graduate School of Life Science and Biotechnology, CHA Research Institute, College of Medicine, Seoul, South Korea.

出版信息

Dev Genes Evol. 2008 Oct;218(10):561-6. doi: 10.1007/s00427-008-0253-9. Epub 2008 Sep 23.

DOI:10.1007/s00427-008-0253-9
PMID:18810488
Abstract

To examine whether the epigenetic status of the human Oct4 promoter is similarly regulated in mouse cells, we engineered a human bacterial artificial chromosome to express green fluorescent protein under the control of the hOct4 promoter and stably integrated it into mouse embryonic stem cells (mESCs), NIH3T3, and 293T cells. The hOct4 promoter is unmethylated in mESCs and it undergoes methylation during retinoic acid-induced differentiation. However, the hOct4 promoter is demethylated in NIH3T3 cells even though it is fully methylated in 293T cells. Methylation status of the hOct4 promoter is associated with green fluorescent protein expression at transcription level. Our findings indicate that the hOct4 promoter is differently regulated in mouse cells.

摘要

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本文引用的文献

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Faithful tissue-specific expression of the human chromosome 21-linked COL6A1 gene in BAC-transgenic mice.人类21号染色体连锁的COL6A1基因在BAC转基因小鼠中的忠实组织特异性表达。
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Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract.胚胎癌细胞提取物对OCT4和NANOG调控区域的表观遗传重编程。
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DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei.DNA去甲基化对于体细胞细胞核的表观遗传重编程是必要的。
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